The transporters SLC35A1 and SLC30A1 play opposite roles in cell survival upon VSV virus infection.
Carcinoma, Non-Small-Cell Lung
/ genetics
Cation Transport Proteins
/ genetics
Genetic Engineering
Host-Pathogen Interactions
Humans
Lung Neoplasms
/ genetics
Nucleotide Transport Proteins
/ genetics
Rhabdoviridae Infections
/ complications
Tumor Cells, Cultured
Vesiculovirus
/ isolation & purification
Virus Replication
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
18 07 2019
18 07 2019
Historique:
received:
29
01
2019
accepted:
04
07
2019
entrez:
20
7
2019
pubmed:
20
7
2019
medline:
27
10
2020
Statut:
epublish
Résumé
Host factor requirements for different classes of viruses have not been fully unraveled. Replication of the viral genome and synthesis of viral proteins within the human host cell are associated with an increased demand for nutrients and specific metabolites. With more than 400 acknowledged members to date in humans, solute carriers (SLCs) represent the largest family of transmembrane proteins dedicated to the transport of ions and small molecules such as amino acids, sugars and nucleotides. Consistent with their impact on cellular metabolism, several SLCs have been implicated as host factors affecting the viral life cycle and the cellular response to infection. In this study, we aimed at characterizing the role of host SLCs in cell survival upon viral infection by performing unbiased genetic screens using a focused CRISPR knockout library. Genetic screens with the cytolytic vesicular stomatitis virus (VSV) showed that the loss of two SLCs genes, encoding the sialic acid transporter SLC35A1/CST and the zinc transporter SLC30A1/ZnT1, affected cell survival upon infection. Further characterization of these genes suggests a role for both of these transporters in the apoptotic response induced by VSV, offering new insights into the cellular response to oncolytic virus infections.
Identifiants
pubmed: 31320712
doi: 10.1038/s41598-019-46952-9
pii: 10.1038/s41598-019-46952-9
pmc: PMC6639343
doi:
Substances chimiques
Cation Transport Proteins
0
Nucleotide Transport Proteins
0
SLC30A1 protein, human
0
SLC35A1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
10471Subventions
Organisme : Austrian Science Fund FWF
ID : P 29250
Pays : Austria
Références
J Virol. 2011 Mar;85(6):2990-3000
pubmed: 21191006
Nat Cell Biol. 2013 Dec;15(12):1473-85
pubmed: 24185178
J Virol. 2010 Feb;84(3):1563-73
pubmed: 19906910
Genome Biol. 2014;15(12):550
pubmed: 25516281
J Biol Chem. 2011 Feb 25;286(8):5935-41
pubmed: 21173156
J Immunol Res. 2018 Jul 5;2018:5214187
pubmed: 30069489
Elife. 2012 Nov 13;1:e00049
pubmed: 23150796
J Virol. 1999 Aug;73(8):6937-45
pubmed: 10400792
Science. 2008 Feb 15;319(5865):921-6
pubmed: 18187620
Biometals. 2001 Sep-Dec;14(3-4):315-30
pubmed: 11831462
Cell Host Microbe. 2018 Jun 13;23(6):766-774.e5
pubmed: 29779931
Cell Struct Funct. 2015;40(1):13-30
pubmed: 25399611
Hum Immunol. 2003 Jan;64(1):82-92
pubmed: 12507817
Science. 2018 Dec 7;362(6419):1171-1177
pubmed: 30442766
Viruses. 2018 Feb 23;10(2):
pubmed: 29473868
Blood. 2005 Apr 1;105(7):2671-6
pubmed: 15576474
Cancer Res. 2014 Jun 15;74(12):3199-204
pubmed: 24830719
Nature. 2011 Aug 24;477(7364):340-3
pubmed: 21866103
Science. 2014 Jan 3;343(6166):84-87
pubmed: 24336571
Cell Rep. 2018 Apr 10;23(2):596-607
pubmed: 29642015
Cell. 2015 Jul 30;162(3):478-87
pubmed: 26232220
Mol Aspects Med. 2013 Apr-Jun;34(2-3):590-600
pubmed: 23506892
Lancet. 2017 Feb 4;389(10068):505-518
pubmed: 28017403
Cell. 2003 Nov 14;115(4):449-59
pubmed: 14622599
Nucleic Acids Res. 2018 Jun 1;46(10):e58
pubmed: 29538768
J Cell Sci. 2010 Oct 1;123(Pt 19):3347-56
pubmed: 20826457
Nat Genet. 2017 Feb;49(2):193-203
pubmed: 27992415
Nat Rev Cancer. 2014 Aug;14(8):559-67
pubmed: 24990523
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W401-7
pubmed: 24861617
J Am Chem Soc. 2001 Aug 15;123(32):7831-41
pubmed: 11493056
Cell Rep. 2016 Oct 25;17(5):1357-1368
pubmed: 27783949
Mol Aspects Med. 2013 Apr-Jun;34(2-3):95-107
pubmed: 23506860
Virology. 2015 May;479-480:609-18
pubmed: 25812764
J Virol. 2012 May;86(9):4868-82
pubmed: 22345471
Cell Death Dis. 2017 Oct 5;8(10):e3069
pubmed: 28981087
Front Neurosci. 2015 Nov 06;9:421
pubmed: 26594142
Biochim Biophys Acta. 2005 Jul 10;1744(3):406-14
pubmed: 15979510
Metallomics. 2014 Sep;6(9):1656-63
pubmed: 24951051
Sci Rep. 2016 Aug 22;6:31782
pubmed: 27545104
Am J Hum Genet. 2013 Apr 4;92(4):632-6
pubmed: 23561849
Science. 2009 Nov 27;326(5957):1231-5
pubmed: 19965467
Protein Sci. 2012 Jul;21(7):1056-65
pubmed: 22573662
J Virol. 2009 Oct;83(20):10761-9
pubmed: 19656871
J Gen Virol. 2014 Feb;95(Pt 2):263-277
pubmed: 24225499
Cell Death Differ. 2018 Mar;25(3):486-541
pubmed: 29362479
J Virol. 2001 Apr;75(7):3474-9
pubmed: 11238874
Sci Rep. 2016 Jan 14;6:19428
pubmed: 26763587
ACS Chem Biol. 2018 May 18;13(5):1279-1290
pubmed: 29364645
Cell Rep. 2017 Aug 15;20(7):1503-1512
pubmed: 28813663
Proc Natl Acad Sci U S A. 2003 Jun 10;100(12):6952-7
pubmed: 12756304
Sci Rep. 2018 Oct 19;8(1):15486
pubmed: 30341327
Mol Aspects Med. 2013 Apr-Jun;34(2-3):548-60
pubmed: 23506888