Integrated Transcriptomic and Proteomic Analysis of Red Blood Cells from Rainbow Trout Challenged with VHSV Point Towards Novel Immunomodulant Targets.
IFIT5
Mx
VHSV
antigen presentation
complement
erythrocytes
interferon
proteome
red blood cells
rhabdoviruses
transcriptome
β-defensin 1
Journal
Vaccines
ISSN: 2076-393X
Titre abrégé: Vaccines (Basel)
Pays: Switzerland
ID NLM: 101629355
Informations de publication
Date de publication:
09 Jul 2019
09 Jul 2019
Historique:
received:
05
06
2019
revised:
29
06
2019
accepted:
01
07
2019
entrez:
21
7
2019
pubmed:
22
7
2019
medline:
22
7
2019
Statut:
epublish
Résumé
Teleost red blood cells (RBCs) are nucleated and therefore can propagate cellular responses to exogenous stimuli. RBCs can mount an immune response against a variety of fish viruses, including the viral septicemia hemorrhagic virus (VHSV), which is one of the most prevalent fish viruses resulting in aquaculture losses. In this work, RBCs from blood and head kidney samples of rainbow trout challenged with VHSV were analyzed via transcriptomic and proteomic analyses. We detected an overrepresentation of differentially expressed genes (DEGs) related to the type I interferon response and signaling in RBCs from the head kidney and related to complement activation in RBCs from blood. Antigen processing and presentation of peptide antigen was overrepresented in RBCs from both tissues. DEGs shared by both tissues showed an opposite expression profile. In summary, this work has demonstrated that teleost RBCs can modulate the immune response during an in vivo viral infection, thus implicating RBCs as cell targets for the development of novel immunomodulants.
Identifiants
pubmed: 31324030
pii: vaccines7030063
doi: 10.3390/vaccines7030063
pmc: PMC6789484
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : H2020 European Research Council
ID : GA639249
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