Label-free chemical imaging flow cytometry by high-speed multicolor stimulated Raman scattering.

Cell Line, Tumor Flow Cytometry / methods Humans Imaging, Three-Dimensional Microalgae / cytology Spectrum Analysis, Raman / methods Staining and Labeling
cancer cells imaging flow cytometry metabolite imaging microalgae stimulated Raman scattering

Journal

Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876

Informations de publication

Date de publication:
06 08 2019
Historique:
pubmed: 22 7 2019
medline: 27 3 2020
entrez: 21 7 2019
Statut: ppublish

Résumé

Combining the strength of flow cytometry with fluorescence imaging and digital image analysis, imaging flow cytometry is a powerful tool in diverse fields including cancer biology, immunology, drug discovery, microbiology, and metabolic engineering. It enables measurements and statistical analyses of chemical, structural, and morphological phenotypes of numerous living cells to provide systematic insights into biological processes. However, its utility is constrained by its requirement of fluorescent labeling for phenotyping. Here we present label-free chemical imaging flow cytometry to overcome the issue. It builds on a pulse pair-resolved wavelength-switchable Stokes laser for the fastest-to-date multicolor stimulated Raman scattering (SRS) microscopy of fast-flowing cells on a 3D acoustic focusing microfluidic chip, enabling an unprecedented throughput of up to ∼140 cells/s. To show its broad utility, we use the SRS imaging flow cytometry with the aid of deep learning to study the metabolic heterogeneity of microalgal cells and perform marker-free cancer detection in blood.

Identifiants

DOI: 10.1073/pnas.1902322116 PMID: 31324741 PMC: PMC6690022
pubmed: 31324741
pii: 1902322116
doi: 10.1073/pnas.1902322116
pmc: PMC6690022
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

15842-15848

Informations de copyright

Copyright © 2019 the Author(s). Published by PNAS.

Déclaration de conflit d'intérêts

Conflict of interest statement: Y.O., Y.S., Y.W., and K.G. are inventors of a patent application covering SRS imaging with a wavelength-switched laser. All other authors declare no conflict of interest.

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Auteurs

Yuta Suzuki (Y)

Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.

Koya Kobayashi (K)

Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.

Yoshifumi Wakisaka (Y)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.

Dinghuan Deng (D)

Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.

Shunji Tanaka (S)

Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.

Chun-Jung Huang (CJ)

Department of Photonics, National Chiao Tung University, 300 Hsinchu, Taiwan.

Cheng Lei (C)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
Institute of Technological Sciences, Wuhan University, 430072 Wuhan, China.

Chia-Wei Sun (CW)

Department of Photonics, National Chiao Tung University, 300 Hsinchu, Taiwan.

Hanqin Liu (H)

Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.

Yasuhiro Fujiwaki (Y)

Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan.

Sangwook Lee (S)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.

Akihiro Isozaki (A)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
ORCID: 0000-0003-0391-9832

Yusuke Kasai (Y)

Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, 464-8601 Nagoya, Japan.

Takeshi Hayakawa (T)

Department of Precision Mechanics, Chuo University, 112-8551 Tokyo, Japan.

Shinya Sakuma (S)

Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, 464-8601 Nagoya, Japan.
ORCID: 0000-0002-4145-7264

Fumihito Arai (F)

Department of Micro-Nano Mechanical Science and Engineering, Nagoya University, 464-8601 Nagoya, Japan.

Kenichi Koizumi (K)

Department of Creative Informatics, The University of Tokyo, 113-0033 Tokyo, Japan.

Hiroshi Tezuka (H)

Department of Creative Informatics, The University of Tokyo, 113-0033 Tokyo, Japan.

Mary Inaba (M)

Department of Creative Informatics, The University of Tokyo, 113-0033 Tokyo, Japan.

Kei Hiraki (K)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.

Takuro Ito (T)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
Japan Science and Technology Agency, 332-0012 Saitama, Japan.

Misa Hase (M)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.

Satoshi Matsusaka (S)

Department of Gastroenterology, Cancer Institute Hospital, Japanese Foundation for Cancer Research, 135-8550 Tokyo, Japan.
Clinical Research and Regional Innovation, Faculty of Medicine, University of Tsukuba, 305-8575 Ibaraki, Japan.

Kiyotaka Shiba (K)

Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, 135-8550 Tokyo, Japan.
ORCID: 0000-0001-6459-0204

Kanako Suga (K)

Division of Protein Engineering, Cancer Institute, Japanese Foundation for Cancer Research, 135-8550 Tokyo, Japan.

Masako Nishikawa (M)

Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 113-0033 Tokyo, Japan.

Masahiro Jona (M)

Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 113-0033 Tokyo, Japan.

Yutaka Yatomi (Y)

Department of Clinical Laboratory Medicine, Graduate School of Medicine, The University of Tokyo, 113-0033 Tokyo, Japan.

Yaxiaer Yalikun (Y)

Center for Biosystems Dynamics Research, RIKEN, 565-0871 Osaka, Japan.

Yo Tanaka (Y)

Center for Biosystems Dynamics Research, RIKEN, 565-0871 Osaka, Japan.
ORCID: 0000-0003-1034-5718

Takeaki Sugimura (T)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
Japan Science and Technology Agency, 332-0012 Saitama, Japan.

Nao Nitta (N)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
Japan Science and Technology Agency, 332-0012 Saitama, Japan.

Keisuke Goda (K)

Department of Chemistry, The University of Tokyo, 113-0033 Tokyo, Japan.
Institute of Technological Sciences, Wuhan University, 430072 Wuhan, China.
Japan Science and Technology Agency, 332-0012 Saitama, Japan.
Department of Bioengineering, University of California, Los Angeles, CA 90095.

Yasuyuki Ozeki (Y)

Department of Electrical Engineering and Information Systems, The University of Tokyo, 113-8656 Tokyo, Japan; ozeki@ee.t.u-tokyo.ac.jp.
ORCID: 0000-0002-9004-0799

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules cultivées › Cellules cancéreuses en culture › Lignée cellulaire tumorale › Label-free chemical imaging flow cytometry by...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Cytophotométrie › Cytométrie en flux › Label-free chemical imaging flow cytometry by...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Label-free chemical imaging flow cytometry by...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Traitement d'image par ordinateur › Imagerie tridimensionnelle › Label-free chemical imaging flow cytometry by...
questionsmedicales.fr › Stades d'organismes › Plancton › Phytoplancton › Microalgues › Label-free chemical imaging flow cytometry by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Analyse spectrale Raman › Label-free chemical imaging flow cytometry by...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques histologiques › Techniques de préparation histocytologique › Coloration et marquage › Label-free chemical imaging flow cytometry by...