Use of synthesized double-stranded gene fragments as qPCR standards for the quantification of antibiotic resistance genes.

Anti-Bacterial Agents / pharmacology Cloning, Molecular DNA, Bacterial / isolation & purification Drug Resistance, Microbial / genetics Environmental Monitoring Feces / microbiology Genes, Bacterial / genetics Molecular Biology / methods Real-Time Polymerase Chain Reaction / methods Reproducibility of Results Sensitivity and Specificity Soil Soil Microbiology Water Microbiology
Antibiotic resistance genes Chemically synthesized gene fragments Gene cloning qPCR

Journal

Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883

Informations de publication

Date de publication:
09 2019
Historique:
received: 07 05 2019
revised: 13 07 2019
accepted: 16 07 2019
pubmed: 22 7 2019
medline: 23 6 2020
entrez: 21 7 2019
Statut: ppublish

Résumé

Pollution of various environmental matrices by antibiotic resistance genes (ARGs) has become a growing threat to human health. For the quantitative analysis of the presence of ARGs, there is a need for sensitive and robust qPCR assays which can detect various genes from different types of DNA extracts. Fourteen ARGs were selected as target genes in this study including: bla

Identifiants

DOI: 10.1016/j.mimet.2019.105670 PMID: 31325465
pubmed: 31325465
pii: S0167-7012(19)30374-4
doi: 10.1016/j.mimet.2019.105670
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0
DNA, Bacterial 0
Soil 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

105670

Informations de copyright

Copyright © 2019 Elsevier B.V. All rights reserved.

Auteurs

Like Xu (L)

Healthy Infrastructure Research Group, Department of Civil, Environmental & Geomatic Engineering, University College London, Gower Street, WC1E 6BT London, UK.

Hannah Chen (H)

Healthy Infrastructure Research Group, Department of Civil, Environmental & Geomatic Engineering, University College London, Gower Street, WC1E 6BT London, UK.

Melisa Canales (M)

Healthy Infrastructure Research Group, Department of Civil, Environmental & Geomatic Engineering, University College London, Gower Street, WC1E 6BT London, UK.

Lena Ciric (L)

Healthy Infrastructure Research Group, Department of Civil, Environmental & Geomatic Engineering, University College London, Gower Street, WC1E 6BT London, UK. Electronic address: l.ciric@ucl.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Use of synthesized double-stranded gene...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Clonage moléculaire › Use of synthesized double-stranded gene...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › ADN bactérien › Use of synthesized double-stranded gene...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Use of synthesized double-stranded gene...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pratiques en santé publique › Surveillance de l'environnement › Use of synthesized double-stranded gene...
questionsmedicales.fr › Liquides et sécrétions biologiques › Fèces › Use of synthesized double-stranded gene...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome microbien › Génome bactérien › Gènes bactériens › Use of synthesized double-stranded gene...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Biologie moléculaire › Use of synthesized double-stranded gene...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › Réaction de polymérisation en chaine en temps réel › Use of synthesized double-stranded gene...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Use of synthesized double-stranded gene...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Statistiques comme sujet › Sensibilité et spécificité › Use of synthesized double-stranded gene...
questionsmedicales.fr › Environnement et santé publique › Environnement › Sol › Use of synthesized double-stranded gene...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Microbiologie du sol › Use of synthesized double-stranded gene...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Microbiologie de l'environnement › Microbiologie de l'eau › Use of synthesized double-stranded gene...