The effect of intrathyroidal versus intraperitoneal bevacizumab on thyroid volume and vasculature flow in a rat model.

Bevacizumab thyroid ultrasound

Journal

Gland surgery
ISSN: 2227-684X
Titre abrégé: Gland Surg
Pays: China (Republic : 1949- )
ID NLM: 101606638

Informations de publication

Date de publication:
Jun 2019
Historique:
entrez: 23 7 2019
pubmed: 23 7 2019
medline: 23 7 2019
Statut: ppublish

Résumé

Several clinical conditions increase thyroid gland vascularity, impacting surgical blood loss. Bevacizumab has been observed to reduce thyroid function, possibly through its effect on gland angiogenesis. This study aimed to determine if bevacizumab has any effect on thyroid vascularity as measured by gland volume and superior thyroid artery (STA) flow velocity in the normal rat thyroid. Sixteen adult female Sprague-Dawley rats were placed under general anesthesia to measure baseline thyroid gland characteristics. A Vevo 2100 high-frequency ultrasound with 40 mHz transducer was used to obtain STA flow measurements and thyroid gland dimensions. Four rats served as controls. Six rats received intrathyroidal (IT) injections and 6 received intraperitoneal (IP) injections of bevacizumab (4-5 mg/kg). After two weeks ultrasound measurements were repeated. Pretreatment animals displayed similar thyroid volume and vascularity. Thyroid volume decreased (62.583 Single systemic administration of bevacizumab appears to decrease thyroid volume without an effect on STA flow, VEGF or CD31 staining. These preliminary findings support further study of pharmacologic intervention in thyroid conditions characterized by increased angiogenesis and vascularity, such as iodine deficiency, Graves disease, and hypothyroidism.

Sections du résumé

BACKGROUND BACKGROUND
Several clinical conditions increase thyroid gland vascularity, impacting surgical blood loss. Bevacizumab has been observed to reduce thyroid function, possibly through its effect on gland angiogenesis. This study aimed to determine if bevacizumab has any effect on thyroid vascularity as measured by gland volume and superior thyroid artery (STA) flow velocity in the normal rat thyroid.
METHODS METHODS
Sixteen adult female Sprague-Dawley rats were placed under general anesthesia to measure baseline thyroid gland characteristics. A Vevo 2100 high-frequency ultrasound with 40 mHz transducer was used to obtain STA flow measurements and thyroid gland dimensions. Four rats served as controls. Six rats received intrathyroidal (IT) injections and 6 received intraperitoneal (IP) injections of bevacizumab (4-5 mg/kg). After two weeks ultrasound measurements were repeated.
RESULTS RESULTS
Pretreatment animals displayed similar thyroid volume and vascularity. Thyroid volume decreased (62.583
CONCLUSIONS CONCLUSIONS
Single systemic administration of bevacizumab appears to decrease thyroid volume without an effect on STA flow, VEGF or CD31 staining. These preliminary findings support further study of pharmacologic intervention in thyroid conditions characterized by increased angiogenesis and vascularity, such as iodine deficiency, Graves disease, and hypothyroidism.

Identifiants

pubmed: 31328099
doi: 10.21037/gs.2018.09.12
pii: gs-08-03-212
pmc: PMC6606475
doi:

Types de publication

Journal Article

Langues

eng

Pagination

212-217

Déclaration de conflit d'intérêts

Conflicts of Interest: Other Presentations—This manuscript was given as a poster presentation at that American Academy of Otolaryngology Head and Neck Surgery Annual Meeting in Chicago, IL, USA.

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Auteurs

Aaron Smith (A)

Department of Otolaryngology, Head & Neck Surgery, University of Tennessee Health Science Center, Memphis, TN, USA.

Vikrum Thimmappa (V)

Department of Otolaryngology, Head & Neck Surgery, University of Tennessee Health Science Center, Memphis, TN, USA.

John D Boughter (JD)

Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, Memphis, TN, USA.

Christopher Vanison (C)

Department of Otolaryngology, Head & Neck Surgery, University of Tennessee Health Science Center, Memphis, TN, USA.

Courtney B Shires (CB)

Department of Otolaryngology, Head & Neck Surgery, University of Tennessee Health Science Center, Memphis, TN, USA.

Merry Sebelik (M)

Department of Otolaryngology, Head & Neck Surgery, Emory University, Atlanta, GA, USA.

Classifications MeSH