Peptides from allergenic lipocalins bind to formyl peptide receptor 3 in human dendritic cells to mediate T
Lipocalin allergens
T(H)2 polarization
antagonistic peptide
formyl peptide receptor 3
human monocyte-derived dendritic cells
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
04
03
2019
revised:
14
06
2019
accepted:
10
07
2019
pubmed:
23
7
2019
medline:
23
9
2020
entrez:
23
7
2019
Statut:
ppublish
Résumé
How T In an in vitro system we compared the effect of lipocalin allergens and nonallergenic homologues on human monocyte-derived dendritic cells (DCs) to investigate how they polarize naive CD4 We examined whether lipocalins and FPR3 colocalize within the cells by using confocal microscopy. With calcium mobilization assays of FPR3-transfected HEK 293 cells, we measured FPR3 signaling in response to allergenic and nonallergenic lipocalins. Silencing of FPR3 in DCs and pretreatment with an antagonistic peptide were used to assess the function of FPR3 in T FPR3 and lipocalins colocalize in the same vesicles in DCs. Cathepsin S-digested allergenic lipocalins, but not digestion products of nonallergenic homologues, activate FPR3 signaling. FPR3 silencing in DCs or pretreatment with an antagonistic peptide restores IL-12 and induces IL-10 expression by DCs treated with lipocalin allergens, attenuating the T We describe a novel molecular mechanism for induction of T
Sections du résumé
BACKGROUND
How T
OBJECTIVE
In an in vitro system we compared the effect of lipocalin allergens and nonallergenic homologues on human monocyte-derived dendritic cells (DCs) to investigate how they polarize naive CD4
METHODS
We examined whether lipocalins and FPR3 colocalize within the cells by using confocal microscopy. With calcium mobilization assays of FPR3-transfected HEK 293 cells, we measured FPR3 signaling in response to allergenic and nonallergenic lipocalins. Silencing of FPR3 in DCs and pretreatment with an antagonistic peptide were used to assess the function of FPR3 in T
RESULTS
FPR3 and lipocalins colocalize in the same vesicles in DCs. Cathepsin S-digested allergenic lipocalins, but not digestion products of nonallergenic homologues, activate FPR3 signaling. FPR3 silencing in DCs or pretreatment with an antagonistic peptide restores IL-12 and induces IL-10 expression by DCs treated with lipocalin allergens, attenuating the T
CONCLUSION
We describe a novel molecular mechanism for induction of T
Identifiants
pubmed: 31330220
pii: S0091-6749(19)30942-X
doi: 10.1016/j.jaci.2019.07.008
pmc: PMC7115912
mid: EMS87710
pii:
doi:
Substances chimiques
Allergens
0
FPR3 protein, human
0
Lipocalins
0
Peptides
0
Receptors, Formyl Peptide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
654-665Subventions
Organisme : Austrian Science Fund FWF
ID : P 27543
Pays : Austria
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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