TAK1 knock-down in macrophage alleviate lung inflammation induced by black carbon and aged black carbon.
Air Pollutants
/ toxicity
Animals
Carbon
/ metabolism
Cytokines
/ metabolism
Female
Gene Knockdown Techniques
Interleukin-1beta
/ metabolism
Interleukin-33
/ metabolism
MAP Kinase Kinase Kinases
/ genetics
Macrophages
/ drug effects
Male
Mice
Mice, Inbred C57BL
NF-kappa B
/ metabolism
Phosphorylation
Pneumonia
/ chemically induced
RAW 264.7 Cells
Soot
/ toxicity
1,4NQ-BC
BC
Lung inflammation
Macrophage
TAK1
Journal
Environmental pollution (Barking, Essex : 1987)
ISSN: 1873-6424
Titre abrégé: Environ Pollut
Pays: England
ID NLM: 8804476
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
16
08
2018
revised:
05
05
2019
accepted:
24
06
2019
pubmed:
23
7
2019
medline:
9
11
2019
entrez:
23
7
2019
Statut:
ppublish
Résumé
Black carbon (BC) can combine with organic matter and form secondary pollutants known as aged BC. BC and aged BC can cause respiratory system inflammation and induce lesions at relevant sites, but the underlying mechanism has remained unknown. To gain insight into the potential mechanisms, we focused on macrophages and transforming growth factor β-activated kinase 1 (TAK1) which are a crucial factor in inflammation. Our research aims to determine the role of TAK1 in macrophages in pulmonary inflammation induced by particulate matter. In this study, BC and 1,4-naphthoquinone were mixed to model aged BC (1,4NQ-BC) in atmosphere. BC induced mice lung inflammation model, lung macrophage knock-down TAK1 animal model and primary macrophage knock-down TAK1 model were used to explore whether TAK1 in macrophage is a critical role in the process of inflammation. The results showed that the expressions of inflammatory cytokines (IL-1β, IL-6, IL-33) mRNA were significantly increased and the phosphorylation of MAPK and NF-κB signaling pathway related proteins were enhanced in RAW 264.7 cell lines. In vivo studies revealed that the indicators of pulmonary inflammation (pathology, inflammatory cell numbers) and related cytokines (IL-1β, IL-6, IL-33) mRNA expressions in CD11c-Map3k7
Identifiants
pubmed: 31330343
pii: S0269-7491(18)33346-3
doi: 10.1016/j.envpol.2019.06.096
pii:
doi:
Substances chimiques
Air Pollutants
0
Cytokines
0
Il33 protein, mouse
0
Interleukin-1beta
0
Interleukin-33
0
NF-kappa B
0
Soot
0
Carbon
7440-44-0
MAP Kinase Kinase Kinases
EC 2.7.11.25
MAP kinase kinase kinase 7
EC 2.7.11.25
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
507-515Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.