Abalone Collagen Extracts Potentiate Stem Cell Properties of Human Epidermal Keratinocytes.
Animals
Cell Line
Cell Movement
/ drug effects
Cell Self Renewal
/ drug effects
Cell Survival
/ drug effects
Collagen
/ chemistry
Epidermal Growth Factor
/ pharmacology
Epidermis
/ drug effects
Gastropoda
/ chemistry
Humans
Keratinocytes
/ drug effects
Molecular Weight
Spheroids, Cellular
/ drug effects
Stem Cells
/ drug effects
MTT
abalone collagen extract
keratinocytes
stemness
wound healing
Journal
Marine drugs
ISSN: 1660-3397
Titre abrégé: Mar Drugs
Pays: Switzerland
ID NLM: 101213729
Informations de publication
Date de publication:
20 Jul 2019
20 Jul 2019
Historique:
received:
12
06
2019
revised:
11
07
2019
accepted:
18
07
2019
entrez:
24
7
2019
pubmed:
25
7
2019
medline:
7
3
2020
Statut:
epublish
Résumé
Stem cell activities in human tissues are critical for tissue integrity and function. Maintaining keratinocyte stem cells (KSCs) stemness helps sustain healthy skin by supporting keratinocyte renewal, involving the formation of epidermal barriers. In this study, abalone collagen (AC) extracts with molecular weights of 3 kDa (AC 1) and 300 kDa (AC 2) were compared to the epidermal growth factor (EGF) for their effects on cell proliferation, cell migration (wound healing), spheroid formation, and the expression level of stem cell markers on human keratinocytes (HaCaT cells). Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and cell proliferation was quantified by ATP and DNA content analysis and Sulforhodamine B (SRB) assays. Cell migration assay was determined using the scratch wound healing test. Spheroid formation was evaluated and the expression level of stem cell markers was investigated by western blot analysis. The results showed that AC 1 at the concentration of 100 µg/mL could stimulate HaCaT cell proliferation, migration, spheroid formation, and the expression level of stem cell markers (keratin 19, β-catenin, ALDH1A1) compared to the control. In conclusion, a smaller molecular weight of abalone collagen extract exhibits a better effect on keratinocytes proliferation, migration, and stemness, which could be a potential active ingredient in cosmeceutical products.
Identifiants
pubmed: 31330853
pii: md17070424
doi: 10.3390/md17070424
pmc: PMC6669461
pii:
doi:
Substances chimiques
Epidermal Growth Factor
62229-50-9
Collagen
9007-34-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Research and Researcher for Industries
ID : MSD60I0092
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