Hyperspectral optical coherence tomography for in vivo visualization of melanin in the retinal pigment epithelium.
hyperspectral imaging
melanin
optical coherence tomography
optical spectroscopy
retinal pigment epithelium
white light
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
24
04
2019
revised:
19
06
2019
accepted:
18
07
2019
pubmed:
25
7
2019
medline:
22
10
2020
entrez:
24
7
2019
Statut:
ppublish
Résumé
Previous studies for melanin visualization in the retinal pigment epithelium (RPE) have exploited either its absorption properties (using photoacoustic tomography or photothermal optical coherence tomography [OCT]) or its depolarization properties (using polarization sensitive OCT). However, these methods are only suitable when the melanin concentration is sufficiently high. In this work, we present the concept of hyperspectral OCT for melanin visualization in the RPE when the concentration is low. Based on white light OCT, a hyperspectral stack of 27 wavelengths (440-700 nm) was created in post-processing for each depth-resolved image. Owing to the size and shape of the melanin granules in the RPE, the variations in backscattering coefficient as a function of wavelength could be identified-a result which is to be expected from Mie theory. This effect was successfully identified both in eumelanin-containing phantoms and in vivo in the low-concentration Brown Norway rat RPE.
Identifiants
pubmed: 31334610
doi: 10.1002/jbio.201900153
pmc: PMC7065636
doi:
Substances chimiques
Melanins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e201900153Subventions
Organisme : European Research Council
ID : 640396
Pays : International
Organisme : Austrian Science Fund (FWF)
ID : P25823-B24
Pays : International
Informations de copyright
© 2019 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Références
J Biophotonics. 2019 Dec;12(12):e201900153
pubmed: 31334610
Biomed Opt Express. 2018 Jul 06;9(8):3581-3589
pubmed: 30338141
Opt Lett. 2007 Dec 15;32(24):3525-7
pubmed: 18087530
Invest Ophthalmol Vis Sci. 2013 Jan 28;54(1):739-45
pubmed: 23258154
Opt Express. 2008 Oct 13;16(21):16410-22
pubmed: 18852747
Opt Lett. 2006 Apr 15;31(8):1079-81
pubmed: 16625909
Sci Rep. 2017 Jun 9;7(1):3150
pubmed: 28600515
J Biol Eng. 2018 Dec 4;12:29
pubmed: 30534199
PLoS One. 2015 Apr 29;10(4):e0125631
pubmed: 25923208
J Opt Soc Am A Opt Image Sci Vis. 2008 Feb;25(2):304-11
pubmed: 18246163
Biomed Opt Express. 2012 Jul 1;3(7):1670-83
pubmed: 22808437
Invest Ophthalmol Vis Sci. 1986 Feb;27(2):145-52
pubmed: 3943941
Opt Lett. 2018 Oct 15;43(20):5162-5165
pubmed: 30320845
Invest Ophthalmol Vis Sci. 2006 Aug;47(8):3556-64
pubmed: 16877429
Nat Photonics. 2011 Dec 1;5(12):744-747
pubmed: 23144652
Invest Ophthalmol Vis Sci. 2008 Jun;49(6):2661-7
pubmed: 18515594
Opt Express. 2009 Jul 20;17(15):13157-67
pubmed: 19654721
Opt Lett. 2013 Jun 1;38(11):1796-8
pubmed: 23722747
Biomed Opt Express. 2015 Mar 24;6(4):1429-50
pubmed: 25909026
Photochem Photobiol. 2008 May-Jun;84(3):639-44
pubmed: 18346089
J Struct Biol. 2010 Aug;171(2):163-73
pubmed: 20450977
Opt Express. 2010 Feb 15;18(4):3967-72
pubmed: 20389409
Biomed Opt Express. 2018 Apr 05;9(5):2115-2129
pubmed: 29760974
Am J Ophthalmol. 2008 Oct;146(4):496-500
pubmed: 18639219
Proc Natl Acad Sci U S A. 2005 Jun 21;102(25):8978-83
pubmed: 15951427
Invest Ophthalmol Vis Sci. 1985 May;26(5):657-69
pubmed: 3997417
Exp Eye Res. 2003 Jan;76(1):89-98
pubmed: 12589778
Invest Ophthalmol Vis Sci. 1986 Jul;27(7):1063-7
pubmed: 3721785
Phys Med Biol. 2002 Aug 21;47(16):2863-77
pubmed: 12222851
Prog Retin Eye Res. 2011 Nov;30(6):431-51
pubmed: 21729763
Opt Express. 2005 Jul 11;13(14):5450-62
pubmed: 19498540
J Biomed Opt. 2015 Oct;20(10):106005
pubmed: 26469564
Biomed Opt Express. 2013 Oct 23;4(11):2570-84
pubmed: 24298417
Opt Lett. 2000 Jan 15;25(2):111-3
pubmed: 18059799
J Biomed Opt. 2018 Sep;23(9):1-16
pubmed: 30264553
Opt Express. 2009 Apr 13;17(8):6799-812
pubmed: 19365509
Exp Eye Res. 2012 May;98:23-7
pubmed: 22440812
Biomed Opt Express. 2018 Jun 28;9(7):3464-3480
pubmed: 29984110
Biomed Opt Express. 2017 May 04;8(6):2851-2865
pubmed: 28663911
Opt Express. 2004 Nov 1;12(22):5487-501
pubmed: 19484110
J Biomed Opt. 2011 Mar;16(3):030503
pubmed: 21456858
Biomed Opt Express. 2018 Apr 04;9(5):2056-2067
pubmed: 29760969
Invest Ophthalmol Vis Sci. 2015 Nov;56(12):7462-72
pubmed: 26595606
Ophthalmic Res. 1993;25(1):65-8
pubmed: 8446370
Biomed Opt Express. 2018 Aug 20;9(9):4372-4389
pubmed: 30615718
Arch Ophthalmol. 1964 Sep;72:380-7
pubmed: 14186782
Pigment Cell Res. 2006 Dec;19(6):572-94
pubmed: 17083485
Sci Rep. 2017 Mar 07;7:42353
pubmed: 28266502
Opt Express. 2004 May 31;12(11):2404-22
pubmed: 19475077
Physiol Rev. 2005 Jul;85(3):845-81
pubmed: 15987797
Transl Vis Sci Technol. 2018 Sep 04;7(5):4
pubmed: 30197836
Biomed Opt Express. 2017 Aug 07;8(9):3966-3980
pubmed: 29026682
Biomed Opt Express. 2014 Aug 25;5(9):3150-9
pubmed: 25401028