Evaluation of reference genes for real-time quantitative PCR analysis in southern corn rootworm, Diabrotica undecimpunctata howardi (Barber).

Animals Coleoptera / genetics Genes, Insect Genotyping Techniques / methods Insect Proteins / genetics Reference Standards Reverse Transcriptase Polymerase Chain Reaction / methods

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 07 2019
Historique:
received: 16 11 2018
accepted: 08 07 2019
entrez: 26 7 2019
pubmed: 26 7 2019
medline: 22 10 2020
Statut: epublish

Résumé

Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated the performance of six southern corn rootworm, Diabrotica undecimpunctata howardi (Barber), housekeeping genes (HKG), β-actin (Actin), β-tubulin (Tubulin), elongation factor 1 alpha (EF1α), glyceraldehyde-3 phosphate dehydrogenase (GAPDH), 40 S ribosomal protein S9 (RpS9) and ubiquitin-conjugating protein (Ubi), under different experimental conditions such as developmental stage, exposure of neonate and adults to dsRNA, exposure of adults to different temperatures, different 3

Identifiants

DOI: 10.1038/s41598-019-47020-y PMID: 31341190 PMC: PMC6656754
pubmed: 31341190
doi: 10.1038/s41598-019-47020-y
pii: 10.1038/s41598-019-47020-y
pmc: PMC6656754
doi:

Substances chimiques

Insect Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

10703

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Auteurs

Saumik Basu (S)

Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Department of Entomology, Washington State University, Pullman, WA, 99164, USA.

Adriano E Pereira (AE)

Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA. pereiraa@missouri.edu.
Plant Genetics Research Unit, USDA/ARS, University of Missouri-Columbia, Columbia, MO, 65211-7020, USA. pereiraa@missouri.edu.
ORCID: 0000-0002-9901-7026

Daniele H Pinheiro (DH)

Federal University of Lavras, Lavras, MG, Brazil.

Haichuan Wang (H)

Department of Agronomy and Horticulture, University of Nebraska-Lincoln, Lincoln, NE, 68583-0915, USA.

Arnubio Valencia-Jiménez (A)

Departamento de Producción Agropecuaria, Universidad de Caldas, Manizales, Colombia.

Blair D Siegfried (BD)

Entomology and Nematology Department, University of Florida, Gainesville, FL, 32611-0620, USA.

Joe Louis (J)

Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.

Xuguo 'Joe' Zhou (X')

Department of Entomology, University of Kentucky, Lexington, KY, 40546-0091, USA.
ORCID: 0000-0002-2385-8224

Ana Maria Vélez (AM)

Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA.
ORCID: 0000-0002-8329-6243

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Evaluation of reference genes for real-time...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Coléoptères › Evaluation of reference genes for real-time...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Génome d'insecte › Gènes d'insecte › Evaluation of reference genes for real-time...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques de génotypage › Evaluation of reference genes for real-time...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Protéines d'insecte › Evaluation of reference genes for real-time...
questionsmedicales.fr › Techniques d'investigation › Poids et mesures › Normes de référence › Evaluation of reference genes for real-time...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Techniques d'amplification d'acides nucléiques › Réaction de polymérisation en chaîne › RT-PCR › Evaluation of reference genes for real-time...