Evaluation of Diagnostic Performance of Three Indirect Enzyme-Linked Immunosorbent Assays for the Detection of IgG Antibodies to Ebola Virus in Human Sera.
Ebola virus
IgG antibody
diagnostic performance
enzyme-linked immunosorbent assay
glycoprotein
human serum
nucleocapsid
whole antigen
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
24 07 2019
24 07 2019
Historique:
received:
29
05
2019
revised:
10
07
2019
accepted:
11
07
2019
entrez:
27
7
2019
pubmed:
28
7
2019
medline:
26
9
2020
Statut:
epublish
Résumé
Filovirus serological diagnosis and epidemiological investigations are hampered due to the unavailability of validated immunoassays. Diagnostic performance of three indirect enzyme-linked immunosorbent assays (I-ELISA) was evaluated for the detection of IgG antibody to Ebola virus (EBOV) in human sera. One I-ELISA was based on a whole EBOV antigen (WAg) and two utilized recombinant nucleocapsid (NP) and glycoproteins (GP), respectively. Validation data sets derived from individual sera collected in South Africa (SA), representing an EBOV non-endemic country, and from sera collected during an Ebola disease (EBOD) outbreak in Sierra Leone (SL), were categorized according to the compounded results of the three I-ELISAs and real time reverse-transcription polymerase chain reaction (RT-PCR). At the cut-off values selected at 95% accuracy level by the two-graph receiver operating characteristic analysis, specificity in the SA EBOV negative serum panel (
Identifiants
pubmed: 31344850
pii: v11080678
doi: 10.3390/v11080678
pmc: PMC6722596
pii:
doi:
Substances chimiques
Antibodies, Viral
0
Antigens, Viral
0
Glycoproteins
0
Immunoglobulin G
0
Nucleocapsid Proteins
0
nucleocapsid protein, Ebola virus
0
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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