HuR interacts with lincBRN1a and lincBRN1b during neuronal stem cells differentiation.
HuR
Neural stem cell
differentiation
long-non-coding RNA
Journal
RNA biology
ISSN: 1555-8584
Titre abrégé: RNA Biol
Pays: United States
ID NLM: 101235328
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
pubmed:
28
7
2019
medline:
1
5
2020
entrez:
27
7
2019
Statut:
ppublish
Résumé
LncRNAs play crucial roles in cellular processes and their regulatory effects in the adult brain and neural stem cells (NSCs) remain to be entirely characterized. We report that 10 lncRNAs (LincENC1, FABL, lincp21, HAUNT, PERIL, lincBRN1a, lincBRN1b, HOTTIP, TUG1 and FENDRR) are expressed during murine NSCs differentiation and interact with the RNA-binding protein ELAVL1/HuR. Furthermore, we characterize the function of two of the deregulated lncRNAs, lincBRN1a and lincBRN1b, during NSCs' differentiation. Their inhibition leads to the induction of differentiation, with a concomitant decrease in stemness and an increase in neuronal markers, indicating that they exert key functions in neuronal cells differentiation. Furthermore, we describe here that HuR regulates their half-life, suggesting their synergic role in the differentiation process. We also identify six human homologs (PANTR1, TUG1, HOTTIP, TP53COR, ELDRR and FENDRR) of the mentioned 10 lncRNAs and we report their deregulation during human iPSCs differentiation into neurons. In conclusion, our results strongly indicate a key synergic role for lncRNAs and HuR in neuronal stem cells fate.
Identifiants
pubmed: 31345103
doi: 10.1080/15476286.2019.1637698
pmc: PMC6779397
doi:
Substances chimiques
Biomarkers
0
ELAV-Like Protein 1
0
Elavl1 protein, mouse
0
RNA, Long Noncoding
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1471-1485Références
Am J Hum Genet. 2012 Dec 7;91(6):1128-34
pubmed: 23217328
Mol Pharmacol. 2016 Feb;89(2):243-52
pubmed: 26677051
Mol Cell. 2011 Sep 16;43(6):904-14
pubmed: 21925379
Curr Pharm Des. 2012;18(13):1755-83
pubmed: 22394166
Cell Stem Cell. 2013 May 2;12(5):616-28
pubmed: 23583100
Genetics. 2012 Nov;192(3):1133-48
pubmed: 22960213
Nat Med. 2008 Jul;14(7):723-30
pubmed: 18587408
Mol Neurobiol. 2015 Oct;52(2):959-69
pubmed: 26055230
Restor Neurol Neurosci. 2017;35(6):583-599
pubmed: 29172009
Mol Cell. 2012 Aug 24;47(4):495-6
pubmed: 22920290
Nature. 2002 Feb 14;415(6873):810-3
pubmed: 11845212
Cell Transplant. 2015;24(4):703-19
pubmed: 25299753
Neurobiol Dis. 2013 Dec;60:51-60
pubmed: 23969235
J Neuroinflammation. 2018 Nov 30;15(1):333
pubmed: 30501635
Cereb Cortex. 2013 Nov;23(11):2632-43
pubmed: 22892427
Nature. 2019 Feb;566(7742):100-104
pubmed: 30700908
Genome Res. 2012 May;22(5):885-98
pubmed: 22406755
Elife. 2013 Dec 31;2:e01749
pubmed: 24381249
Nature. 1996 Jan 11;379(6561):131-7
pubmed: 8538762
Adv Exp Med Biol. 2010;700:106-23
pubmed: 21627034
Mol Ther. 2013 Mar;21(3):503-5
pubmed: 23449106
Nature. 1989 Aug 10;340(6233):471-3
pubmed: 2755510
Biochim Biophys Acta. 2011 Sep;1813(9):1663-7
pubmed: 21315776
Histol Histopathol. 2010 Oct;25(10):1331-40
pubmed: 20712017
Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):2074-7
pubmed: 8446631
Cell. 2007 Jun 29;129(7):1311-23
pubmed: 17604720
Mol Cell. 2014 Feb 6;53(3):506-14
pubmed: 24440503
Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11879-83
pubmed: 8524867
Trends Genet. 2015 May;31(5):239-51
pubmed: 25869999
Postepy Hig Med Dosw (Online). 2005;59:290-8
pubmed: 15995596
Nature. 2011 Apr 7;472(7341):120-4
pubmed: 21423168
Mol Cell Neurosci. 2013 Sep;56:447-55
pubmed: 23439364
BMC Neurosci. 2010 Feb 05;11:14
pubmed: 20137068
Cell Tissue Res. 2018 Jan;371(1):55-71
pubmed: 29079882
Mol Cell Biol. 2016 Apr 15;36(9):1332-41
pubmed: 26884465
J Vis Exp. 2014 Dec 17;(94):null
pubmed: 25548937
Proc Natl Acad Sci U S A. 2015 Jun 2;112(22):6855-62
pubmed: 26034286
Curr Drug Targets. 2015;16(5):499-515
pubmed: 25706256
Nat Cell Biol. 2012 Jun 10;14(7):659-65
pubmed: 22684254
Neurosci Lett. 2016 Jun 20;625:70-9
pubmed: 26733304
J Mol Neurosci. 2013 Mar;49(3):589-93
pubmed: 22949041
Science. 2002 Feb 22;295(5559):1528-32
pubmed: 11859196
Nat Rev Neurosci. 2002 May;3(5):401-9
pubmed: 11988779
Cell Stem Cell. 2014 Jun 5;14(6):752-61
pubmed: 24905165
Cell. 2013 Mar 14;152(6):1298-307
pubmed: 23498938
Neurobiol Dis. 2011 Jul;43(1):86-98
pubmed: 21324364
J Neurosci. 2002 Jan 15;22(2):437-45
pubmed: 11784788
J Adv Res. 2014 May;5(3):277-94
pubmed: 25685495
Nucleic Acids Res. 2011 Jan;39(Database issue):D301-8
pubmed: 21036867
Nucleic Acids Res. 2017 Sep 19;45(16):9514-9527
pubmed: 28934484
Mol Psychiatry. 2014 Apr;19(4):410-6
pubmed: 24468823
Brain Res Brain Res Rev. 2005 Apr;48(2):211-9
pubmed: 15850660
Mol Cell. 2012 Aug 24;47(4):648-55
pubmed: 22841487
Neuropharmacology. 2017 Jun;119:76-90
pubmed: 28373075
ASN Neuro. 2016 Oct 27;8(5):
pubmed: 27789613
Sci Rep. 2015 Nov 10;5:16478
pubmed: 26553968