Effects of laser irradiation on growth factors and cell apoptosis of in vitro cultured infant hemangioma endothelial cells.
Apoptosis
/ radiation effects
Cell Proliferation
Cytokines
/ genetics
Endothelial Cells
/ radiation effects
Fibroblast Growth Factor 2
/ genetics
Gene Expression Regulation, Neoplastic
/ radiation effects
Hemangioma
/ metabolism
Humans
In Vitro Techniques
Infant
Laser Therapy
/ methods
Tumor Cells, Cultured
Vascular Endothelial Growth Factor A
/ genetics
Vascular Endothelial Growth Factor Receptor-2
/ genetics
Cell apoptosis
Growth factors
In vitro
Infant hemangioma
Laser
Journal
Life sciences
ISSN: 1879-0631
Titre abrégé: Life Sci
Pays: Netherlands
ID NLM: 0375521
Informations de publication
Date de publication:
15 Sep 2019
15 Sep 2019
Historique:
received:
30
05
2019
revised:
15
07
2019
accepted:
22
07
2019
pubmed:
28
7
2019
medline:
1
10
2019
entrez:
27
7
2019
Statut:
ppublish
Résumé
The present study aimed to investigate the effects of laser irradiation on the growth factors and cell apoptosis of in vitro cultured infant hemangioma endothelial cells. Endothelial cells of infant hemangioma were cultured in vitro and irradiated using a variable pulse width 1064 nm Nd:YAG laser and intense pulsed light (IPL), the expression of VEGF, VEGFR-2, bFGF and their mRNAs before and after irradiation were measured by ELISA, western blot, RT-PCR and flow cytometry, and changes in the apoptotic rate of endothelial cells in hemangioma were monitored. The mRNA and protein expressions of VEGF, VEGFR-2, bFGF in hemangioma endothelial cells were inhibited by both Nd:YAG laser and ILP compared to the control cells. The apoptotic rates of hemangioma endothelial cells were also decreased after both laser irradiation treatments in comparison to the blank group. The differences were statistically significant. Laser irradiation treats hemangioma not only through a selective photothermal mechanism, but also through cytokine signaling pathways.
Identifiants
pubmed: 31348947
pii: S0024-3205(19)30611-3
doi: 10.1016/j.lfs.2019.116685
pii:
doi:
Substances chimiques
Cytokines
0
VEGFA protein, human
0
Vascular Endothelial Growth Factor A
0
Fibroblast Growth Factor 2
103107-01-3
KDR protein, human
EC 2.7.10.1
Vascular Endothelial Growth Factor Receptor-2
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
116685Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.