Polysaccharide deriving from Ophiopogonis Radix promotes metabolism of ginsenosides in the present of human gut microbiota based on UPLC-MS/MS assay.
Ginsenosides
Human gut microbiota
Ophiopogon polysaccharide
Promoting effects
UPLC-MS/MS
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
25 Oct 2019
25 Oct 2019
Historique:
received:
27
05
2019
revised:
09
07
2019
accepted:
16
07
2019
pubmed:
28
7
2019
medline:
28
1
2020
entrez:
27
7
2019
Statut:
ppublish
Résumé
The combined usage of Ginseng Radix et Rhizoma (ginseng) and Ophiopogonis Radix is common in oriental countries for thousands of years. The major active constituents of ginseng are ginsenosides, and the conversion of ginsenosides to different metabolites by gut microbiota has been reported. However, the effect of Ophiopogonis Radix, especially its polysaccharides, on the metabolism of ginsenosides by gut microbiota is not known. Here, an in vitro metabolism of ginseng extract, or ginsenosides, in combination with or without Ophiopogon polysaccharide was conducted. A sensitive and reliable UPLC-MS/MS approach using multiple reaction monitoring (MRM) in positive ion mode was developed simultaneously to quantify 22 ginsenosides in the broth of gut microbiota. After fermentation with the microbiota, 15 ginsenosides were detected and quantified, including 6 primary ginsenosides, i.e. Rb
Identifiants
pubmed: 31349212
pii: S0731-7085(19)31311-1
doi: 10.1016/j.jpba.2019.112779
pii:
doi:
Substances chimiques
Ginsenosides
0
Polysaccharides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112779Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.