Surface chemistry of gold nanoparticles determines interactions with bovine serum albumin.
Bovine serum albumin
Gold nanoparticles
Interaction
Protein corona
Journal
Materials science & engineering. C, Materials for biological applications
ISSN: 1873-0191
Titre abrégé: Mater Sci Eng C Mater Biol Appl
Pays: Netherlands
ID NLM: 101484109
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
24
03
2019
revised:
01
06
2019
accepted:
01
06
2019
entrez:
28
7
2019
pubmed:
28
7
2019
medline:
31
12
2019
Statut:
ppublish
Résumé
Protein coronas provide a novel technique for the bio identification of nanoparticles in physiological environments, to further elucidate the biological effects of nanoparticles in biomedical applications. Herein, we investigated the adsorption of bovine serum albumin (BSA) on gold nanoparticles (AuNPs) with different surface modifications (citrate, cysteine, polyethylene glycol (PEG), and cetyltrimethylammonium bromide (CTAB)) using UV-vis absorption spectroscopy, fluorescence spectroscopy, circular dichroism (CD), Fourier transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), and transmission electron microscopy (TEM) techniques. It was revealed that the binding of AuNPs modified with citrate, cysteine, PEG (2k), and CTAB to BSA, appeared to be of the static quenching type, with binding constants in the range of from 10
Identifiants
pubmed: 31349396
pii: S0928-4931(19)31101-4
doi: 10.1016/j.msec.2019.109856
pii:
doi:
Substances chimiques
Serum Albumin, Bovine
27432CM55Q
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109856Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.