Carbon nanotube composite hydrogels for vocal fold tissue engineering: Biocompatibility, rheology, and porosity.
Hydrogels;Vocal folds
Tissue engineering;Carbon nanotubes
Journal
Materials science & engineering. C, Materials for biological applications
ISSN: 1873-0191
Titre abrégé: Mater Sci Eng C Mater Biol Appl
Pays: Netherlands
ID NLM: 101484109
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
10
08
2018
revised:
01
06
2019
accepted:
03
06
2019
entrez:
28
7
2019
pubmed:
28
7
2019
medline:
31
12
2019
Statut:
ppublish
Résumé
Porous composite hydrogels were prepared using glycol chitosan as the matrix, glyoxal as the chemical crosslinker, and carbon nanotubes (CNTs) as the fibers. Both carboxylic and hydroxylic functionalized CNTs were used. The homogeneity of CNTs dispersion was evaluated using scanning electron microscopy. Human vocal fold fibroblasts were cultured and encapsulated in the composite hydrogels with different CNT concentrations to quantify cell viability. Rheological tests were performed to determine the gelation time and the storage modulus as a function of CNT concentration. The gelation time tended to decrease for low concentrations and increase at higher concentrations, reaching a local minimum value. The storage modulus obeyed different trends depending on the functional group. The porosity of the hydrogels was found to increase by 120% when higher concentrations of carboxylic CNTs were used. A high porosity may promote cell adhesion, migration, and recruitment from the surrounding native tissue, which will be investigated in a future work aiming at applying this injectable biomaterial for vocal fold tissue regeneration.
Identifiants
pubmed: 31349421
pii: S0928-4931(18)32032-0
doi: 10.1016/j.msec.2019.109861
pmc: PMC6662640
mid: NIHMS1531593
pii:
doi:
Substances chimiques
Hydrogels
0
Nanotubes, Carbon
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109861Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC005788
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
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