Antitumor bioactivity of porphyran extracted from Pyropia yezoensis Chonsoo2 on human cancer cell lines.
Antineoplastic Agents
/ isolation & purification
Cell Cycle
/ drug effects
Cell Line, Tumor
Cell Proliferation
/ drug effects
Cell Survival
/ drug effects
Cyclin B1
/ genetics
Gene Expression Regulation, Neoplastic
/ drug effects
Humans
Plant Extracts
/ isolation & purification
Rhodophyta
/ chemistry
Sepharose
/ analogs & derivatives
Tumor Suppressor Protein p53
/ genetics
Pyropia yezoensis Chonsoo2
antitumor
cell cycle
porphyran
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
19
05
2019
revised:
16
07
2019
accepted:
22
07
2019
pubmed:
28
7
2019
medline:
22
11
2019
entrez:
28
7
2019
Statut:
ppublish
Résumé
Pyropia yezoensis, rich in porphyran, is a medicine-edible red alga. In the present study, the physicochemical characteristics, conformational states and antitumor activities of a novel porphyran extracted from the high-yield algal strain Pyropia yezoensis Chonsoo2 and its two degraded derivatives by gamma irradiation were investigated. Pyropia yezoensis porphyran is a water-soluble, triple-helical sulfated hetero-galactopyranose, named PYP. PYP was degraded by gamma irradiation at 20 kGy and 50 kGy, giving two low molecular weight derivatives comprising PYP-20 and PYP-50, respectively. PYP with a higher molecular weight has a solution conformation different from PYP-20 and PYP-50. Three porphyrans had no toxicity in normal human liver cells (HL-7702) and showed antitumor effects on Hep3B, HeLa and MDA-MB-231. They had better antitumor against HeLa cells, exhibiting a similar inhibition ratio compared to 5-fluorouracil, with PYP especially exhibiting a higher inhibition ratio than 5-fluorouracil. With respect to HeLa cells, the different antitumor activities might be related to porphyran molecular weight and solution conformation. Furthermore, the HeLa cell cycle was blocked in the G2/M phase after PYP treatment, leading to cell proliferation inhibition. The induction of cell cycle arrest was related to the changes in the expression of p21, p53, Cyclin B1 and cyclin-dependent kinase 1. Pyropia yezoensis porphyran, as applied to medicine and functional food, could potentially be used as a non-toxic natural adjuvant in cancer therapy. © 2019 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Pyropia yezoensis, rich in porphyran, is a medicine-edible red alga. In the present study, the physicochemical characteristics, conformational states and antitumor activities of a novel porphyran extracted from the high-yield algal strain Pyropia yezoensis Chonsoo2 and its two degraded derivatives by gamma irradiation were investigated.
RESULTS
RESULTS
Pyropia yezoensis porphyran is a water-soluble, triple-helical sulfated hetero-galactopyranose, named PYP. PYP was degraded by gamma irradiation at 20 kGy and 50 kGy, giving two low molecular weight derivatives comprising PYP-20 and PYP-50, respectively. PYP with a higher molecular weight has a solution conformation different from PYP-20 and PYP-50. Three porphyrans had no toxicity in normal human liver cells (HL-7702) and showed antitumor effects on Hep3B, HeLa and MDA-MB-231. They had better antitumor against HeLa cells, exhibiting a similar inhibition ratio compared to 5-fluorouracil, with PYP especially exhibiting a higher inhibition ratio than 5-fluorouracil. With respect to HeLa cells, the different antitumor activities might be related to porphyran molecular weight and solution conformation. Furthermore, the HeLa cell cycle was blocked in the G2/M phase after PYP treatment, leading to cell proliferation inhibition. The induction of cell cycle arrest was related to the changes in the expression of p21, p53, Cyclin B1 and cyclin-dependent kinase 1.
CONCLUSION
CONCLUSIONS
Pyropia yezoensis porphyran, as applied to medicine and functional food, could potentially be used as a non-toxic natural adjuvant in cancer therapy. © 2019 Society of Chemical Industry.
Substances chimiques
Antineoplastic Agents
0
Cyclin B1
0
Plant Extracts
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
porphyran
11016-36-7
Sepharose
9012-36-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6722-6730Subventions
Organisme : Golden Seed Project, Ministry of Oceans and Fisheries
ID : 213008-05-2-SB910
Organisme : National Natural Science Foundation of China
ID : 41876197
Organisme : National Natural Science Foundation of China
ID : 81872952
Organisme : Natural Science Foundation of Zhejiang Province
ID : LGN18C020004
Organisme : Natural Science Foundation of Zhejiang Province
ID : LY18C020006
Organisme : Scientific Foundation of Education Department of Zhejiang Province
ID : Y201737374
Informations de copyright
© 2019 Society of Chemical Industry.
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