Reduced humidity experienced by mice in vivo coincides with reduced outflow facility measured ex vivo.
C75BL/6J mice
Environmental conditions
Housing
Outflow facility
Relative humidity
Temperature
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
13
06
2019
revised:
05
07
2019
accepted:
23
07
2019
pubmed:
28
7
2019
medline:
15
2
2020
entrez:
28
7
2019
Statut:
ppublish
Résumé
Mice are routinely used to study aqueous humour dynamics. However, physical factors such as temperature and hydration affect outflow facility in enucleated eyes. This retrospective study examined whether differences in temperature and relative humidity experienced by living mice within their housing environment in vivo coincide with differences in outflow facility measured ex vivo. Facility data and environmental records were collected for one enucleated eye from 116 mice (C57BL/6J males, 9-15 weeks old) at two institutions. Outflow facility was reduced when relative humidity was below the lower limit of 45% recommended by the UK Code of Practice, but there was no detectable effect of temperature on outflow facility. Even when accounting for effects of humidity, there were differences in outflow facility measured between institutions and between individual researchers at the same institution. These data indicate that humidity, as well as additional environmental factors experienced by living mice within their housing environment, may significantly affect outflow facility measured ex vivo.
Identifiants
pubmed: 31351057
pii: S0014-4835(19)30438-5
doi: 10.1016/j.exer.2019.107745
pmc: PMC7296948
mid: NIHMS1596519
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107745Subventions
Organisme : NEI NIH HHS
ID : R01 EY022359
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
Références
Singapore Med J. 1999 May;40(5):345-8
pubmed: 10489493
J Ocul Pharmacol Ther. 2016 Oct;32(8):486-487
pubmed: 26982549
Biomed Hub. 2017 Nov 17;2(3):1-10
pubmed: 31988919
Invest Ophthalmol Vis Sci. 2015 Dec;56(13):8331-9
pubmed: 26720486
Exp Eye Res. 2015 Dec;141:15-22
pubmed: 26051991
Korean J Ophthalmol. 1996 Jun;10(1):29-33
pubmed: 8755199
Exp Eye Res. 2009 Apr;88(4):752-9
pubmed: 18977348
Invest Ophthalmol Vis Sci. 2013 Dec 19;54(13):8251-8
pubmed: 24235015
PLoS One. 2016 Mar 07;11(3):e0150694
pubmed: 26949939
Invest Ophthalmol Vis Sci. 2011 Feb 03;52(2):685-94
pubmed: 20861483
Exp Eye Res. 2003 Mar;76(3):349-59
pubmed: 12573664
Invest Ophthalmol Vis Sci. 2014 May 15;55(6):3727-36
pubmed: 24833737
Ophthalmology. 2013 Apr;120(4):724-30
pubmed: 23357622
Invest Ophthalmol Vis Sci. 2015 Sep;56(10):5764-76
pubmed: 26325415
Invest Ophthalmol Vis Sci. 2012 Aug 24;53(9):5838-45
pubmed: 22807298
Medicine (Baltimore). 2016 Aug;95(31):e4453
pubmed: 27495076
Am J Ophthalmol. 2011 May;151(5):792-798.e1
pubmed: 21310379
Exp Eye Res. 2004 Mar;78(3):639-51
pubmed: 15106944
Sci Rep. 2018 Apr 11;8(1):5848
pubmed: 29643342
Clin Exp Ophthalmol. 2015 Nov;43(8):749-64
pubmed: 25950246
J Ophthalmol. 2015;2015:568509
pubmed: 26491552
Invest Ophthalmol Vis Sci. 2014 Jul 15;55(8):4922-33
pubmed: 25028360
J Am Assoc Lab Anim Sci. 2016;55(5):525-33
pubmed: 27657706
Mol Metab. 2018 Jan;7:161-170
pubmed: 29122558
Invest Ophthalmol Vis Sci. 2008 Apr;49(4):1386-91
pubmed: 18385054
PLoS One. 2015 Feb 20;10(2):e0117503
pubmed: 25699675