Continuous WNT Control Enables Advanced hPSC Cardiac Processing and Prognostic Surface Marker Identification in Chemically Defined Suspension Culture.

Bioreactors CD13 Antigens / genetics Cell Culture Techniques / methods Cell Differentiation / drug effects Culture Media / chemistry Humans Mesoderm / metabolism Myocytes, Cardiac / cytology Pluripotent Stem Cells / cytology Receptor Tyrosine Kinase-like Orphan Receptors / genetics Wnt Signaling Pathway / drug effects
ROR1 WNT cardiac differentiation cardiomyocyte chemically defined process human pluripotent stem cells process development stirred tank bioreactor suspension culture upscaling

Journal

Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300

Informations de publication

Date de publication:
13 08 2019
Historique:
received: 20 12 2018
revised: 21 06 2019
accepted: 24 06 2019
pubmed: 30 7 2019
medline: 24 4 2020
entrez: 30 7 2019
Statut: ppublish

Résumé

Aiming at clinical translation, robust directed differentiation of human pluripotent stem cells (hPSCs), preferentially in chemically defined conditions, is a key requirement. Here, feasibility of suspension culture based hPSC-cardiomyocyte (hPSC-CM) production in low-cost, xeno-free media compatible with good manufacturing practice standards is shown. Applying stirred tank bioreactor systems at increasing dimensions, our advanced protocol enables routine production of about 1 million hPSC-CMs/mL, yielding ∼1.3 × 10

Identifiants

DOI: 10.1016/j.stemcr.2019.06.004 PMID: 31353227 PMC: PMC6700605
pubmed: 31353227
pii: S2213-6711(19)30230-9
doi: 10.1016/j.stemcr.2019.06.004
pmc: PMC6700605
pii:
doi:

Substances chimiques

Culture Media 0
ROR1 protein, human EC 2.7.10.1
Receptor Tyrosine Kinase-like Orphan Receptors EC 2.7.10.1
CD13 Antigens EC 3.4.11.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

366-379

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

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Auteurs

Caroline Halloin (C)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Kristin Schwanke (K)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Wiebke Löbel (W)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Annika Franke (A)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Monika Szepes (M)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Santoshi Biswanath (S)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Stephanie Wunderlich (S)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Sylvia Merkert (S)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Natalie Weber (N)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.

Felix Osten (F)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.

Jeanne de la Roche (J)

Institute for Neurophysiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Felix Polten (F)

Division of Molecular and Translational Cardiology and Department of Cardiology and Angiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Kai Christoph Wollert (K)

Division of Molecular and Translational Cardiology and Department of Cardiology and Angiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Theresia Kraft (T)

Institute of Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625, Hannover, Germany.

Martin Fischer (M)

Institute for Neurophysiology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Ulrich Martin (U)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Ina Gruh (I)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany.

Henning Kempf (H)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Electronic address: henningkempf@outlook.com.

Robert Zweigerdt (R)

Leibniz Research Laboratories for Biotechnology and Artificial Organs (LEBAO), Department of Cardiac, Thoracic-, Transplantation and Vascular Surgery, REBIRTH-Cluster of Excellence, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 Hannover, Germany. Electronic address: zweigerdt.robert@mh-hannover.de.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Biotechnologie › Bioréacteurs › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes de différenciation des lymphocytes T › Antigènes CD13 › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Structures de l'embryon › Feuillets embryonnaires › Mésoderme › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Cellules › Cellules musculaires › Myocytes cardiaques › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs à activité tyrosine kinase › Récepteurs orphelins de type récepteur à tyrosine kinase › Continuous WNT Control Enables Advanced hPSC...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Transduction du signal › Voie de signalisation Wnt › Continuous WNT Control Enables Advanced hPSC...