Temporal association of the development of oropharyngeal microbiota with early life wheeze in a population-based birth cohort.
Granulicatella
Microbiome
Neisseria
Wheeze
Journal
EBioMedicine
ISSN: 2352-3964
Titre abrégé: EBioMedicine
Pays: Netherlands
ID NLM: 101647039
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
03
05
2019
revised:
12
07
2019
accepted:
13
07
2019
pubmed:
30
7
2019
medline:
16
1
2020
entrez:
30
7
2019
Statut:
ppublish
Résumé
A critical window in infancy has been proposed, during which the microbiota may affect subsequent health. The longitudinal development of the oropharyngeal microbiota is under-studied and may be associated with early-life wheeze. We aimed to investigate the temporal association of the development of the oropharyngeal microbiota with early-life wheeze. A population-based birth cohort based in London, UK was followed for 24 months. We collected oropharyngeal swabs at six time-points. Microbiota was determined using sequencing of the V3-V5 region of the 16S rRNA-encoding gene. Medical records were reviewed for the outcome of doctor diagnosed wheeze. We used a time-varying model to investigate the temporal association between the development of microbiota and doctor-diagnosed wheeze. 159 participants completed the study to 24 months and for 98 there was complete sequencing data at all timepoints and outcome data. Of these, 26 had doctor-diagnosed wheeze. We observed significant increase in the abundance of Neisseria between 9 and 24 months in children who developed wheeze (p = 0∙003), while in those without wheezing there was a significant increment in the abundance of Granulicatella (p = 0∙012) between 9 and 12 months, and of Prevotella (p = 0∙018) after 18 months. A temporal association between the respiratory commensal Granulicatella and also Prevotella with wheeze (negative), and between Neisseria and wheeze (positive) was identified in infants prior to one year of age. This adds to evidence for the proposed role of the microbiota in the development of wheeze. FUND: Research funding from the Winnicott Foundation, Meningitis Now and Micropathology Ltd.
Sections du résumé
BACKGROUND
BACKGROUND
A critical window in infancy has been proposed, during which the microbiota may affect subsequent health. The longitudinal development of the oropharyngeal microbiota is under-studied and may be associated with early-life wheeze. We aimed to investigate the temporal association of the development of the oropharyngeal microbiota with early-life wheeze.
METHODS
METHODS
A population-based birth cohort based in London, UK was followed for 24 months. We collected oropharyngeal swabs at six time-points. Microbiota was determined using sequencing of the V3-V5 region of the 16S rRNA-encoding gene. Medical records were reviewed for the outcome of doctor diagnosed wheeze. We used a time-varying model to investigate the temporal association between the development of microbiota and doctor-diagnosed wheeze.
FINDINGS
RESULTS
159 participants completed the study to 24 months and for 98 there was complete sequencing data at all timepoints and outcome data. Of these, 26 had doctor-diagnosed wheeze. We observed significant increase in the abundance of Neisseria between 9 and 24 months in children who developed wheeze (p = 0∙003), while in those without wheezing there was a significant increment in the abundance of Granulicatella (p = 0∙012) between 9 and 12 months, and of Prevotella (p = 0∙018) after 18 months.
INTERPRETATION
CONCLUSIONS
A temporal association between the respiratory commensal Granulicatella and also Prevotella with wheeze (negative), and between Neisseria and wheeze (positive) was identified in infants prior to one year of age. This adds to evidence for the proposed role of the microbiota in the development of wheeze. FUND: Research funding from the Winnicott Foundation, Meningitis Now and Micropathology Ltd.
Identifiants
pubmed: 31353293
pii: S2352-3964(19)30476-1
doi: 10.1016/j.ebiom.2019.07.034
pmc: PMC6710983
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
486-498Informations de copyright
Copyright © 2019. Published by Elsevier B.V.
Références
PLoS One. 2013 Apr 22;8(4):e61217
pubmed: 23630581
Am J Respir Crit Care Med. 2016 Nov 1;194(9):1104-1115
pubmed: 27135599
J Allergy Clin Immunol. 2018 Nov;142(5):1447-1456.e9
pubmed: 29330010
Am J Respir Crit Care Med. 2017 Dec 15;196(12):1582-1590
pubmed: 28665684
Clin Exp Allergy. 2014 Jun;44(6):842-50
pubmed: 24330256
PLoS One. 2012;7(3):e32543
pubmed: 22470420
Am J Respir Crit Care Med. 2014 Dec 1;190(11):1283-92
pubmed: 25329446
Allergy. 2018 Sep;73(9):1918-1921
pubmed: 29862523
Mediators Inflamm. 2017;2017:5047403
pubmed: 29445257
J Allergy Clin Immunol. 2018 Jul;142(1):86-95
pubmed: 29452199
PLoS One. 2010 Jan 05;5(1):e8578
pubmed: 20052417
N Engl J Med. 2007 Oct 11;357(15):1487-95
pubmed: 17928596
ISME J. 2015 May;9(5):1246-59
pubmed: 25575312
J Allergy Clin Immunol. 2017 Mar;139(3):826-834.e13
pubmed: 27576124
Nat Rev Immunol. 2010 Dec;10(12):861-8
pubmed: 21060319
EBioMedicine. 2016 Jul;9:336-345
pubmed: 27333043
Cell Host Microbe. 2015 May 13;17(5):704-15
pubmed: 25865368
Appl Environ Microbiol. 2012 Sep;78(17):6262-70
pubmed: 22752171
Am J Respir Crit Care Med. 2014 Jun 1;189(11):1309-15
pubmed: 24702670
Nucleic Acids Res. 2007;35(21):7188-96
pubmed: 17947321
PLoS One. 2012;7(10):e46803
pubmed: 23071640
EMBO Rep. 2012 May 01;13(5):440-7
pubmed: 22422004
Allergy. 2018 Oct;73(10):2000-2011
pubmed: 29602225
Nat Methods. 2013 Dec;10(12):1200-2
pubmed: 24076764
Sci Rep. 2017 Sep 12;7(1):11266
pubmed: 28900158
Arch Dis Child. 2004 Jun;89(6):540-3
pubmed: 15155399
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4578-85
pubmed: 20668239
PLoS Comput Biol. 2014 Apr 03;10(4):e1003531
pubmed: 24699258
Nat Methods. 2010 May;7(5):335-6
pubmed: 20383131
Appl Environ Microbiol. 2007 Jan;73(1):278-88
pubmed: 17071787
Am J Respir Crit Care Med. 2013 Nov 15;188(10):1246-52
pubmed: 24090102
Expert Rev Clin Immunol. 2017 Jul;13(7):705-713
pubmed: 27817211
Annu Rev Physiol. 2016;78:481-504
pubmed: 26527186
Biotechnol Bioeng. 2005 Mar 20;89(6):670-9
pubmed: 15696537