Insulin acutely increases glucose transporter 1 on plasma membranes and glucose uptake in an AKT-dependent manner in chicken adipocytes.

Adipocytes / drug effects Animals Biological Transport / drug effects Cell Membrane / drug effects Chickens / metabolism Deoxyglucose / metabolism Glucose / metabolism Glucose Transporter Type 1 / metabolism Insulin / pharmacology Male Phosphorylation / drug effects Proto-Oncogene Proteins c-akt / metabolism
AKT Adipocyte Aves Chicken Glucose transporter Insulin

Journal

General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735

Informations de publication

Date de publication:
01 11 2019
Historique:
received: 15 02 2019
revised: 21 06 2019
accepted: 25 07 2019
pubmed: 30 7 2019
medline: 19 2 2020
entrez: 30 7 2019
Statut: ppublish

Résumé

Avian glucose transporters (GLUT) responsible for insulin-responsive glucose uptake into adipocytes remain poorly characterized. We aimed to identify the insulin-responsive GLUT using primary culture of chicken adipocytes. Acute stimulation with 1 μM insulin for 20 min increased 2-deoxyglucose uptake, AKT protein phosphorylation, and GLUT1 protein levels on the plasma membrane of the chicken adipocytes, whereas pretreatment with 10 μM triciribine, an AKT inhibitor, canceled these effects. Furthermore, the insulin stimulation did not affect GLUT12 protein levels on the plasma membrane of the chicken adipocytes. Our results suggest that GLUT1 is an insulin-responsive GLUT in chicken adipocytes.

Identifiants

DOI: 10.1016/j.ygcen.2019.113232 PMID: 31356813
pubmed: 31356813
pii: S0016-6480(19)30087-5
doi: 10.1016/j.ygcen.2019.113232
pii:
doi:

Substances chimiques

Glucose Transporter Type 1 0
Insulin 0
Deoxyglucose 9G2MP84A8W
Proto-Oncogene Proteins c-akt EC 2.7.11.1
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

113232

Informations de copyright

Copyright © 2019. Published by Elsevier Inc.

Auteurs

Saki Shimamoto (S)

Department of Biochemical Science and Technology, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Kazuki Nakashima (K)

Division of Animal Metabolism and Nutrition, Institute of Livestock and Grassland Science, NARO, 2 Ikenodai, Tsukuba 305-0901, Japan.

Ryo Kamimura (R)

Department of Biochemical Science and Technology, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Rukana Kohrogi (R)

Department of Biochemical Science and Technology, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Hiroki Inoue (H)

Department of Biochemical Science and Technology, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Nao Nishikoba (N)

Department of Biochemical Science and Technology, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Akira Ohtsuka (A)

Department of Biochemical Science and Technology, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan.

Daichi Ijiri (D)

Department of Biochemical Science and Technology, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; The United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan. Electronic address: ijiri@chem.agri.kagoshima-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Cellules › Cellules du tissu conjonctif › Adipocytes › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Eucaryotes › Animaux › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Métabolisme › Transport biologique › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Oiseaux › Poulets › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Glucides › Désoses › Désoxyglucose › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Protéines transporteurs de solutés › Transporteurs de glucose par diffusion facilitée › Transporteur de glucose de type 1 › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Hormones peptidiques › Hormones pancréatiques › Insulines › Insuline › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Métabolisme › Phosphorylation › Insulin acutely increases glucose transporter...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines tumorales › Protéines oncogènes › Protéines proto-oncogènes › Protéines proto-oncogènes c-akt › Insulin acutely increases glucose transporter...