INNO-LiPA DNA line probe assay misidentification of M. smegmatis as Mycobacterium fortuitum complex.


Journal

Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899

Informations de publication

Date de publication:
Nov 2019
Historique:
received: 08 01 2019
revised: 26 05 2019
accepted: 20 06 2019
pubmed: 31 7 2019
medline: 28 2 2020
entrez: 31 7 2019
Statut: ppublish

Résumé

Seven weeks after being kicked in the face by a cow, a 34-year-old male patient developed a posttraumatic mycobacterial lymphadenitis. A rapidly growing mycobacterial isolate cultured from a surgically drained lymphadenitis pus specimen was identified as Mycobacterium smegmatis by matrix-assisted laser desorption/ionization mass spectrometry and a combination of ITS-, hsp65-, and 16S rRNA-DNA sequence analysis, but as Mycobacterium fortuitum complex using the commercial INNO-LiPA Mycobacteria v2 line probe assay. As it is unclear if the misidentification of this strain is an exception, more research is required.

Identifiants

pubmed: 31358343
pii: S0732-8893(18)30787-9
doi: 10.1016/j.diagmicrobio.2019.06.010
pii:
doi:

Substances chimiques

Reagent Kits, Diagnostic 0

Types de publication

Case Reports Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

114858

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Theo van den Broek (T)

Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands. Electronic address: t.vandenbroek-2@umcutrecht.nl.

Nard G Janssen (NG)

Department of Oral and Maxillofacial Surgery, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.

David J Hetem (DJ)

Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands; Department of Medical Microbiology, Haaglanden Medical Center, 2501, CK, The Hague, The Netherlands.

Wouter Bekers (W)

Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.

Miranda Kamst (M)

National Institute for Public Health and the Environment (RIVM), 3720, BA, Bilthoven, The Netherlands.

Ad C Fluit (AC)

Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.

Jakko van Ingen (J)

Radboudumc Center for Infectious Diseases, Department of Medical Microbiology, Radboud University Medical Center, 6500, HB, Nijmegen, The Netherlands.

Johannes G Kusters (JG)

Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.

Rob J Rentenaar (RJ)

Department of Medical Microbiology, University Medical Center Utrecht, 3508, GA, Utrecht, The Netherlands.

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Classifications MeSH