Targeted insertion of large DNA sequences by homology-directed repair or non-homologous end joining in engineered tobacco BY-2 cells using designed zinc finger nucleases.
DNA recombination
electroporation
gene targeting
genome editing
particle bombardment
split marker genes
zinc finger nucleases
Journal
Plant direct
ISSN: 2475-4455
Titre abrégé: Plant Direct
Pays: England
ID NLM: 101716131
Informations de publication
Date de publication:
Jul 2019
Jul 2019
Historique:
received:
10
04
2019
revised:
11
06
2019
accepted:
03
07
2019
entrez:
31
7
2019
pubmed:
31
7
2019
medline:
31
7
2019
Statut:
epublish
Résumé
Targeted integration of recombinant DNA fragments into plant genomes by DNA double-strand break (DSB) repair mechanisms has become a powerful tool for precision engineering of crops. However, many targeting platforms require the screening of many transgenic events to identify a low number of targeted events among many more random insertion events. We developed an engineered transgene integration platform (ETIP) that uses incomplete marker genes at the insertion site to enable rapid phenotypic screening and recovery of targeted events upon functional reconstitution of the marker genes. The two marker genes, encoding neomycin phosphotransferase II (
Identifiants
pubmed: 31360827
doi: 10.1002/pld3.153
pii: PLD3153
pmc: PMC6639735
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e00153Déclaration de conflit d'intérêts
K.J., R.B., P.M., P.S., D.R.C, S.R.W., D.O.G., W.M.A., and O.F. were employed by Dow AgroSciences LLC, a wholly owned subsidiary of The Dow Chemical Company, at the time the work was performed, and Dow AgroSciences provided funding for the research. Dow AgroSciences is now part of Corteva Agrisciences, which has filed a patent application on the ETIP concept and the cell lines containing the targeting cassette. All authors declare no conflict of interest.
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