Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
27
02
2019
accepted:
11
07
2019
entrez:
31
7
2019
pubmed:
31
7
2019
medline:
19
2
2020
Statut:
epublish
Résumé
Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)9 and BP100CH7, were investigated for delivery efficiency into rice callus. Both BP100(KH)9 and BP100CH7 successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus.
Identifiants
pubmed: 31361745
doi: 10.1371/journal.pone.0214033
pii: PONE-D-19-05740
pmc: PMC6667096
doi:
Substances chimiques
Bacterial Proteins
0
Cell-Penetrating Peptides
0
Drug Carriers
0
Ions
0
Lipid Bilayers
0
Luminescent Proteins
0
Oligopeptides
0
citrine protein, bacteria
0
lysyl-lysyl-leucyl-phenylalanyl-lysyl-lysyl-isoleucyl-leucyl-lysyl-tyrosyl-leucinamide
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0214033Déclaration de conflit d'intérêts
The authors have a collaboration with BASF SE. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
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