A protein-RNA interaction atlas of the ribosome biogenesis factor AATF.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 07 2019
30 07 2019
Historique:
received:
03
01
2019
accepted:
19
07
2019
entrez:
1
8
2019
pubmed:
1
8
2019
medline:
24
10
2020
Statut:
epublish
Résumé
AATF is a central regulator of the cellular outcome upon p53 activation, a finding that has primarily been attributed to its function as a transcription factor. Recent data showed that AATF is essential for ribosome biogenesis and plays a role in rRNA maturation. AATF has been implicated to fulfil this role through direct interaction with rRNA and was identified in several RNA-interactome capture experiments. Here, we provide a first comprehensive analysis of the RNA bound by AATF using CLIP-sequencing. Interestingly, this approach shows predominant binding of the 45S pre-ribosomal RNA precursor molecules. Furthermore, AATF binds to mRNAs encoding for ribosome biogenesis factors as well as snoRNAs. These findings are complemented by an in-depth analysis of the protein interactome of AATF containing a large set of proteins known to play a role in rRNA maturation with an emphasis on the protein-RNA-complexes known to be required for the generation of the small ribosomal subunit (SSU). In line with this finding, the binding sites of AATF within the 45S rRNA precursor localize in close proximity to the SSU cleavage sites. Consequently, our multilayer analysis of the protein-RNA interactome of AATF reveals this protein to be an important hub for protein and RNA interactions involved in ribosome biogenesis.
Identifiants
pubmed: 31363146
doi: 10.1038/s41598-019-47552-3
pii: 10.1038/s41598-019-47552-3
pmc: PMC6667500
doi:
Substances chimiques
AATF protein, human
0
Apoptosis Regulatory Proteins
0
RNA Precursors
0
Repressor Proteins
0
Ribosomal Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11071Subventions
Organisme : U.S. Department of Health & Human Services | National Institutes of Health (NIH)
ID : HG007005 and NS075449
Pays : International
Organisme : U.S. Department of Health & Human Services | NIH | National Human Genome Research Institute (NHGRI)
ID : U54HG007005
Pays : International
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