Thesaurus: quantifying phosphopeptide positional isomers.
HeLa Cells
Humans
Insulin Receptor Substrate Proteins
/ metabolism
Isomerism
MCF-7 Cells
Phosphatidylinositol 3-Kinases
/ metabolism
Phosphopeptides
/ analysis
Phosphoproteins
/ analysis
Phosphorylation
Proteome
/ analysis
Proto-Oncogene Proteins c-akt
/ metabolism
Search Engine
/ methods
Signal Transduction
Tandem Mass Spectrometry
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
03
10
2017
accepted:
19
06
2019
entrez:
1
8
2019
pubmed:
1
8
2019
medline:
9
11
2019
Statut:
ppublish
Résumé
Proteins can be phosphorylated at neighboring sites resulting in different functional states, and studying the regulation of these sites has been challenging. Here we present Thesaurus, a search engine that detects and quantifies phosphopeptide positional isomers from parallel reaction monitoring and data-independent acquisition mass spectrometry experiments. We apply Thesaurus to analyze phosphorylation events in the PI3K/AKT signaling pathway and show neighboring sites with distinct regulation.
Identifiants
pubmed: 31363206
doi: 10.1038/s41592-019-0498-4
pii: 10.1038/s41592-019-0498-4
pmc: PMC7012383
mid: NIHMS1532385
doi:
Substances chimiques
IRS1 protein, human
0
Insulin Receptor Substrate Proteins
0
Phosphopeptides
0
Phosphoproteins
0
Proteome
0
Phosphatidylinositol 3-Kinases
EC 2.7.1.-
AKT1 protein, human
EC 2.7.11.1
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
703-706Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103533
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA192983
Pays : United States
Organisme : NHGRI NIH HHS
ID : RM1 HG010461
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM119536
Pays : United States
Organisme : NHGRI NIH HHS
ID : U54 HG008097
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG056359
Pays : United States
Organisme : NIGMS NIH HHS
ID : F31 GM119273
Pays : United States
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