High-resolution and high-accuracy topographic and transcriptional maps of the nucleosome barrier.
E. coli
epigenetic modifications
high-resolution optical tweezers
histone ubiquitination
histone variant H2A.Z
human
molecular biophysics
nucleosome transcription
structural biology
transcription regulation
xenopus
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
31 07 2019
31 07 2019
Historique:
received:
08
05
2019
accepted:
30
07
2019
pubmed:
1
8
2019
medline:
31
1
2020
entrez:
1
8
2019
Statut:
epublish
Résumé
Nucleosomes represent mechanical and energetic barriers that RNA Polymerase II (Pol II) must overcome during transcription. A high-resolution description of the barrier topography, its modulation by epigenetic modifications, and their effects on Pol II nucleosome crossing dynamics, is still missing. Here, we obtain topographic and transcriptional (Pol II residence time) maps of canonical, H2A.Z, and monoubiquitinated H2B (uH2B) nucleosomes at near base-pair resolution and accuracy. Pol II crossing dynamics are complex, displaying pauses at specific loci, backtracking, and nucleosome hopping between wrapped states. While H2A.Z widens the barrier, uH2B heightens it, and both modifications greatly lengthen Pol II crossing time. Using the dwell times of Pol II at each nucleosomal position we extract the energetics of the barrier. The orthogonal barrier modifications of H2A.Z and uH2B, and their effects on Pol II dynamics rationalize their observed enrichment in +1 nucleosomes and suggest a mechanism for selective control of gene expression.
Identifiants
pubmed: 31364986
doi: 10.7554/eLife.48281
pii: 48281
pmc: PMC6744274
doi:
pii:
Substances chimiques
Histones
0
Nucleosomes
0
RNA Polymerase II
EC 2.7.7.-
Banques de données
Dryad
['10.5061/dryad.8sb6h8n']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01GM098401
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM098401
Pays : United States
Organisme : Alfred P. Sloan Foundation
ID : FG-2018-10394
Pays : International
Organisme : NIGMS NIH HHS
ID : R01 GM032543
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01GM097260
Pays : United States
Organisme : Burroughs Wellcome Fund
ID : Collaborative Research Travel Grant
Pays : International
Organisme : NIGMS NIH HHS
ID : R01 GM097260
Pays : United States
Organisme : Basic Energy Sciences
ID : Nanomachine Program under Contract DE-AC02-05CH11231
Pays : International
Organisme : NIGMS NIH HHS
ID : R01GM071552
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM071552
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01GM032543
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2019, Chen et al.
Déclaration de conflit d'intérêts
ZC, RG, AB, AL, AS, CD, CK, EK, TY, CB No competing interests declared
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