Whole genome sequencing revealed microbiome in lung adenocarcinomas presented as ground-glass nodules.
Lung cancer
ground-glass nodule (GGN)
microbiome
whole genome sequencing (WGS)
Journal
Translational lung cancer research
ISSN: 2218-6751
Titre abrégé: Transl Lung Cancer Res
Pays: China
ID NLM: 101646875
Informations de publication
Date de publication:
Jun 2019
Jun 2019
Historique:
entrez:
2
8
2019
pubmed:
2
8
2019
medline:
2
8
2019
Statut:
ppublish
Résumé
Emerging evidence has suggested that dysbiosis of the microbiota may play vital roles in tumorigenesis. However, the interplay between the microbiome and lung cancer remains undetermined. In this study, we characterize the microbiome in the early stage of lung cancer, which presented as ground-glass nodules (GGNs). We sequenced the whole genomes from 10 GGN lesions and 5 adjacent normal lung tissue samples. After being filtered with human genome sequences, the sequence reads were mapped to prokaryotic genomes refSeq and non-redundant protein database for taxa and gene functions profiling, respectively. Mycobacterium, Corynebacterium, and Negativicoccus were the core microbiota found in all GGNs and the normal tissue samples. The microbiota composition did not show significant difference between GGNs and normal tissues except the adenocarcinoma (AD) (P=0.047). A significant β diversity in microbiome gene functions was found among different patients. Two individual gene functions, the Secondary Metabolism (1.32 fold with P=0.01) and the Serine Threonine protein kinase (4.23 fold, P<0.001), were significantly increased in GGNs over normal tissue samples. This study helps shed light on the implication of the microbiome in early stage lung cancer, which encourages the further study and development of innovative strategies for early prevention and treatment of lung cancer.
Sections du résumé
BACKGROUND
BACKGROUND
Emerging evidence has suggested that dysbiosis of the microbiota may play vital roles in tumorigenesis. However, the interplay between the microbiome and lung cancer remains undetermined. In this study, we characterize the microbiome in the early stage of lung cancer, which presented as ground-glass nodules (GGNs).
METHODS
METHODS
We sequenced the whole genomes from 10 GGN lesions and 5 adjacent normal lung tissue samples. After being filtered with human genome sequences, the sequence reads were mapped to prokaryotic genomes refSeq and non-redundant protein database for taxa and gene functions profiling, respectively.
RESULTS
RESULTS
Mycobacterium, Corynebacterium, and Negativicoccus were the core microbiota found in all GGNs and the normal tissue samples. The microbiota composition did not show significant difference between GGNs and normal tissues except the adenocarcinoma (AD) (P=0.047). A significant β diversity in microbiome gene functions was found among different patients. Two individual gene functions, the Secondary Metabolism (1.32 fold with P=0.01) and the Serine Threonine protein kinase (4.23 fold, P<0.001), were significantly increased in GGNs over normal tissue samples.
CONCLUSIONS
CONCLUSIONS
This study helps shed light on the implication of the microbiome in early stage lung cancer, which encourages the further study and development of innovative strategies for early prevention and treatment of lung cancer.
Identifiants
pubmed: 31367537
doi: 10.21037/tlcr.2019.06.11
pii: tlcr-08-03-235
pmc: PMC6626866
doi:
Types de publication
Journal Article
Langues
eng
Pagination
235-246Déclaration de conflit d'intérêts
Conflicts of Interest: The authors have no conflicts of interest to declare.
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