Imaging and spatial analysis of hematopoietic stem cell niches.
bone marrow microenvironment
hematopoietic stem cell niches
multidimensional imaging
spatial statistics
stromal cells
Journal
Annals of the New York Academy of Sciences
ISSN: 1749-6632
Titre abrégé: Ann N Y Acad Sci
Pays: United States
ID NLM: 7506858
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
30
03
2019
revised:
30
05
2019
accepted:
06
06
2019
pubmed:
2
8
2019
medline:
2
10
2020
entrez:
2
8
2019
Statut:
ppublish
Résumé
Hematopoietic stem cells (HSCs) have been long proposed to reside in defined anatomical locations within bone marrow (BM) tissues in direct contact or close proximity to nurturing cell types. Imaging techniques that allow the simultaneous mapping of HSCs and interacting cell types have been central to the discovery of basic principles of these so-called HSC niches. Despite major progress in the field, a quantitative and comprehensive model of the cellular and molecular components that define these specialized microenvironments is lacking to date, and uncertainties remain on the preferential localization of HSCs in the context of complex BM tissue landscapes. Recent technological breakthroughs currently allow for the quantitative spatial analysis of BM cellular components with extraordinary precision. Here, we critically discuss essential technical aspects related to imaging approaches, image processing tools, and spatial statistics, which constitute the three basic elements of rigorous quantitative spatial analyses of HSC niches in the BM microenvironment.
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
5-16Informations de copyright
© 2019 New York Academy of Sciences.
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