Phagocytosis by macrophages depends on histamine H2 receptor signaling and scavenger receptor 1.
Atg12
Beclin-1
Hrh2
Msr1
autophagy
bacteria
innate immunity
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
16
05
2019
revised:
19
06
2019
accepted:
30
06
2019
pubmed:
2
8
2019
medline:
23
6
2020
entrez:
2
8
2019
Statut:
ppublish
Résumé
The histamine H2 receptor (H2R) is a G protein-coupled receptor that mediates cyclic AMP production, protein kinase A activation, and MAP kinase signaling. In order to explore the multifaceted effects of histamine signaling on immune cells, phagocytosis was evaluated using primary mouse-derived macrophages. Phagocytosis is initiated by signaling via surface-bound scavenger receptors and can be regulated by autophagy. Absence of H2R signaling resulted in diminished phagocytosis of live bacteria and synthetic microspheres by primary macrophages from histamine H2 receptor gene (Hrh2)-deficient mice. Flow cytometry and immunofluorescence microscopy were used to quantify phagocytosis of phylogenetically diverse bacteria as well as microspheres of defined chemical composition. Autophagy and scavenger receptor gene expression were quantified in macrophages after exposure to Escherichia coli. Expression of the autophagy genes, Becn1 and Atg12, was increased in Hrh2
Identifiants
pubmed: 31369218
doi: 10.1002/mbo3.908
pmc: PMC6813435
doi:
Substances chimiques
Hrh2 protein, mouse
0
Msr1 protein, mouse
0
Receptors, Histamine H2
0
Scavenger Receptors, Class A
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e908Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK056338
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA170930
Pays : United States
Informations de copyright
© 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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