Colorimetric determination of the activity of methyltransferase based on nicking enzyme amplification and the use of gold nanoparticles conjugated to graphene oxide.
Crosslinking effect
Drug development
Hairpin DNA
M. SssI activity
Nanomaterial
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
01 08 2019
01 08 2019
Historique:
received:
23
04
2019
accepted:
07
07
2019
entrez:
3
8
2019
pubmed:
3
8
2019
medline:
3
8
2019
Statut:
epublish
Résumé
A method is described for the colorimetric determination of the activity of CpG methyltransferase (M.SssI). It is based on (a) the crosslinking effect between dsDNA-modified gold nanoparticles (AuNPs) and graphene oxide (GO), and (b) an amplification reaction with the aid of a nicking enzyme. To avoid the aggregation of AuNPs (which would produce false signals), a hairpin DNA was connected to the AuNPs. Thus, the red color of the solution (measured at 530 nm) increases linearly with the activity of M.SssI from 0.2 to 60 U·mL
Identifiants
pubmed: 31372831
doi: 10.1007/s00604-019-3690-4
pii: 10.1007/s00604-019-3690-4
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
594Subventions
Organisme : National Natural Science Foundation of China
ID : 21365015
Pays : International
Organisme : National Natural Science Foundation of China
ID : 21605075
Pays : International
Organisme : Natural Science Foundation of Jiangxi Province (CN)
ID : 20181BAB203020
Pays : International
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