Analysis of thrombin-antithrombin complex formation using microchip electrophoresis and mass spectrometry.
Biomarkers
Disease diagnosis
Dot blot
Point-of-care diagnostics
Protein surface modification
Journal
Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
28
06
2019
accepted:
29
07
2019
pubmed:
3
8
2019
medline:
31
3
2020
entrez:
3
8
2019
Statut:
ppublish
Résumé
Preterm birth (PTB) related health problems take over one million lives each year, and currently, no clinical analysis is available to determine if a fetus is at risk for PTB. Here, we describe the preparation of a key PTB risk biomarker, thrombin-antithrombin (TAT), and characterize it using dot blots, MS, and microchip electrophoresis (µCE). The pH for fluorescently labeling TAT was also optimized using spectrofluorometry and spectrophotometry. The LOD of TAT was measured in µCE. Lastly, TAT was combined with six other PTB risk biomarkers and separated in µCE. The ability to make and characterize TAT is an important step toward the development of an integrated microfluidic diagnostic for PTB risk.
Identifiants
pubmed: 31373007
doi: 10.1002/elps.201900235
pmc: PMC6829041
mid: NIHMS1046555
doi:
Substances chimiques
Biomarkers
0
antithrombin III-protease complex
0
Antithrombin III
9000-94-6
Peptide Hydrolases
EC 3.4.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2853-2859Subventions
Organisme : Brigham Young University for the use of the Integrated Microfabrication Lab facilities
Pays : International
Organisme : NIH HHS
ID : R01 EB027096
Pays : United States
Organisme : Undergraduate Research Assistantship from Brigham Young University
Pays : International
Organisme : NIBIB NIH HHS
ID : R01 EB027096
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB006124
Pays : United States
Organisme : NIH HHS
ID : R01 EB006124
Pays : United States
Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Références
Electrophoresis. 2017 Jul;38(13-14):1743-1754
pubmed: 28272749
Anal Bioanal Chem. 2018 Jan;410(3):933-941
pubmed: 28799040
Anal Chem. 2003 Apr 15;75(8):1941-5
pubmed: 12713054
J Biol Chem. 1997 Aug 1;272(31):19393-400
pubmed: 9235938
Am J Obstet Gynecol. 2001 Sep;185(3):643-51
pubmed: 11568793
Electrophoresis. 2018 Sep;39(18):2300-2307
pubmed: 29683528
Anal Bioanal Chem. 2019 Aug;411(21):5405-5413
pubmed: 30382326
J Pharm Sci. 2017 Apr;106(4):973-981
pubmed: 28007564
J Biol Chem. 1982 Dec 25;257(24):14891-5
pubmed: 7174671
N Engl J Med. 1997 Sep 4;337(10):688-98
pubmed: 9278467
Anal Chem. 2019 Jun 4;91(11):7418-7425
pubmed: 31056901
J Mater Chem B. 2014 Sep 21;2(35):5718-5738
pubmed: 32262016
Am J Obstet Gynecol. 2011 May;204(5):391.e1-8
pubmed: 21074133
Nat Methods. 2009 May;6(5):359-62
pubmed: 19377485
Eur J Vasc Endovasc Surg. 2003 May;25(5):432-7
pubmed: 12713782