Immunoassays are not immune to errors: Examples from two studies of steroid output from freshwater trout farms.

Animals Aquaculture Enzyme-Linked Immunosorbent Assay Fresh Water Immunoassay / methods Radioimmunoassay Reference Standards Reproducibility of Results Rivers Steroids / analysis Trout / immunology Water / chemistry
Calculation error Enzyme-linked immunoassay Extraction Radioimmunoassay Reproducibility crisis Steroid

Journal

General and comparative endocrinology
ISSN: 1095-6840
Titre abrégé: Gen Comp Endocrinol
Pays: United States
ID NLM: 0370735

Informations de publication

Date de publication:
01 01 2020
Historique:
received: 28 03 2019
revised: 27 06 2019
accepted: 18 07 2019
pubmed: 3 8 2019
medline: 11 3 2020
entrez: 3 8 2019
Statut: ppublish

Résumé

A "reproducibility crisis" is widespread across scientific disciplines, where results and conclusions of studies are not supported by subsequent investigation. Here we provide a steroid immunoassay example where human errors generated unreproducible results and conclusions. Our study was triggered by a scientific report citing abnormally high concentrations (means of 4-79 ng L

Identifiants

DOI: 10.1016/j.ygcen.2019.113226 PMID: 31374286
pubmed: 31374286
pii: S0016-6480(19)30158-3
doi: 10.1016/j.ygcen.2019.113226
pii:
doi:

Substances chimiques

Steroids 0
Water 059QF0KO0R

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

113226

Informations de copyright

Crown Copyright © 2019. Published by Elsevier Inc. All rights reserved.

Auteurs

Tim Ellis (T)

Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK. Electronic address: tim.ellis@cefas.co.uk.

Luigi Margiotta-Casaluci (L)

Brunel University London, Institute for Environment, Health and Societies, Uxbridge, Middlesex UB8 3PH, UK.

Tom G Pottinger (TG)

Centre for Ecology & Hydrology, Fish Ecophysiology, Lake Ecosystem Group, Lancaster Environment Centre, Library Avenue, Bailrigg, Lancaster LA1 4AP, UK.

Steve Morris (S)

Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.

R Allan Reese (RA)

Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.

John P Sumpter (JP)

Brunel University London, Institute for Environment, Health and Societies, Uxbridge, Middlesex UB8 3PH, UK.

Alexander P Scott (AP)

Centre for Environment, Fisheries and Aquaculture Science (Cefas), Weymouth Laboratory, Barrack Road, The Nothe, Weymouth, Dorset DT4 8UB, UK.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Immunoassays are not immune to errors:...
questionsmedicales.fr › Technologie, industrie et agriculture › Agriculture › Aquaculture › Immunoassays are not immune to errors:...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Techniques d'immunoadsorption › Test ELISA › Immunoassays are not immune to errors:...
questionsmedicales.fr › Environnement et santé publique › Environnement › Eau douce › Immunoassays are not immune to errors:...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunoassays are not immune to errors:...
questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Dosage radioimmunologique › Immunoassays are not immune to errors:...
questionsmedicales.fr › Techniques d'investigation › Poids et mesures › Normes de référence › Immunoassays are not immune to errors:...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Méthodologie en recherche épidémiologique › Reproductibilité des résultats › Immunoassays are not immune to errors:...
questionsmedicales.fr › Environnement et santé publique › Environnement › Eau douce › Rivières › Immunoassays are not immune to errors:...
questionsmedicales.fr › Composés polycycliques › Composés à cycles fusionnés › Stéroïdes › Immunoassays are not immune to errors:...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Poissons › Salmoniformes › Salmonidae › Truite › Immunoassays are not immune to errors:...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Eau › Immunoassays are not immune to errors:...