A novel assay for measuring recombinant human lysophosphatidylcholine acyltransferase 3 activity.
LPCAT3
enzyme kinetics
inhibitor screening
lysophosphatidylcholine acyltransferase 3
sequential mechanism
Journal
FEBS open bio
ISSN: 2211-5463
Titre abrégé: FEBS Open Bio
Pays: England
ID NLM: 101580716
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
04
06
2019
revised:
02
07
2019
accepted:
02
08
2019
pubmed:
4
8
2019
medline:
6
5
2020
entrez:
4
8
2019
Statut:
ppublish
Résumé
Lysophosphatidylcholine acyltransferase 3 (LPCAT3) is an important enzyme in phospholipid remodeling, a process that influences the biophysical properties of cell membranes and thus cell function. Multiple lines of evidence suggest that LPCAT3 is involved in several diseases, including atherosclerosis, non-alcoholic steatohepatitis, and carcinoma. Thus, LPCAT3 may have potential as a therapeutic target for these diseases. In the present study, we devised an assay based on reversed-phase HPLC to measure LPCAT3 activity, which may facilitate the identification of LPCAT3 inhibitors and activators. We found that optimal pH and temperature of recombinant human LPCAT3 are 6.0 and 30 °C, respectively. The enzyme K
Identifiants
pubmed: 31376210
doi: 10.1002/2211-5463.12712
pmc: PMC6768109
doi:
Substances chimiques
Enzyme Inhibitors
0
Recombinant Proteins
0
1-Acylglycerophosphocholine O-Acyltransferase
EC 2.3.1.23
LPCAT3 protein, human
EC 2.3.1.23
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1734-1743Informations de copyright
© 2019 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.
Références
J Biol Chem. 2008 Mar 28;283(13):8258-65
pubmed: 18195019
J Lipid Res. 2009 Aug;50(8):1563-70
pubmed: 19351971
Gene. 2011 Jan 1;470(1-2):7-11
pubmed: 20837115
J Biol Chem. 2012 Jun 8;287(24):20122-31
pubmed: 22511767
Arterioscler Thromb Vasc Biol. 2013 Jun;33(6):1171-9
pubmed: 23580142
J Biochem. 2013 Jul;154(1):21-8
pubmed: 23698096
J Lipid Res. 2014 Apr;55(4):782-91
pubmed: 24563510
J Lipid Res. 2014 Jul;55(7):1386-96
pubmed: 24850807
J Cell Biochem. 2015 Dec;116(12):2840-8
pubmed: 25994902
Gastroenterology. 2015 Nov;149(6):1519-29
pubmed: 26226572
J Biol Chem. 2016 Apr 1;291(14):7651-60
pubmed: 26828064
J Biol Chem. 2017 Jan 20;292(3):884-897
pubmed: 27913621
J Vasc Res. 2017;54(4):200-208
pubmed: 28683445
Cell Stem Cell. 2018 Feb 1;22(2):206-220.e4
pubmed: 29395055
Biochim Biophys Acta Mol Cell Biol Lipids. 2018 Aug;1863(8):834-843
pubmed: 29673706
Atherosclerosis. 2018 Aug;275:409-418
pubmed: 29866392
J Gastroenterol Hepatol. 2019 Jul;34(7):1256-1262
pubmed: 30160786
Annu Rev Physiol. 2019 Feb 10;81:165-188
pubmed: 30379616
Front Cardiovasc Med. 2019 Jan 17;5:192
pubmed: 30705887