Metabolomic changes and polyunsaturated fatty acid biosynthesis during gonadal growth and development in the sea urchin Strongylocentrotus intermedius.
Fatty acid
Gonad growth and development
Metabolomics
Strongylocentrotus intermedius
Journal
Comparative biochemistry and physiology. Part D, Genomics & proteomics
ISSN: 1878-0407
Titre abrégé: Comp Biochem Physiol Part D Genomics Proteomics
Pays: Netherlands
ID NLM: 101270611
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
22
03
2019
revised:
10
06
2019
accepted:
24
07
2019
pubmed:
4
8
2019
medline:
3
4
2020
entrez:
4
8
2019
Statut:
ppublish
Résumé
Strongylocentrotus intermedius is an important commercial species of sea urchin distributed in the coastal waters of China. However, the metabolomic changes that accompany its gonadal growth and development stages remain unclear. In this study, we have histologically observed gonad growth stages, analyzed the fatty acid composition, and employed an untargeted metabolomic approach to investigate the metabolites associated with the gonadal growth and development of S. intermedius, as well as the biosynthesis and metabolism of polyunsaturated fatty acids (PUFAs) at different stages and in different sexes. The gonad mass of sea urchin increased from 0.70 ± 0.18 g in January (at the recovering stage) to 8.78 ± 2.89 g in July (the reproductive stage), with the GSI increasing from 4.02 ± 0.88% to 16.86 ± 2.79%. We have analyzed 34 types of fatty acids, such as arachidonic acid, eicosapentaenoic acid, etc., of which PUFAs were the dominant fatty acid class in this species, accounting for >48.55% of the total. In the metabolomic analysis, linolelaidic acid, sciadonic acid, cis-8,11,14,17-eicosatetraenoic acid, adrenic acid, docosapentaenoic acid, and tetracosapentaenoic acid were detected in the differentially expressed metabolites of the unsaturated fatty acids biosynthesis pathway. We found that the most significant functional pathways during gonadal growth and development were "arachidonic acid metabolism", "alpha-linolenic acid metabolism" and "linoleic acid metabolism", which are all related to fatty acid metabolism. These results will provide valuable information on the possible presence of both exogenous and endogenous fatty acids in sea urchin gonads and the metabolomic changes in S. intermedius during gonadal growth periods, and will further our understanding of the intermediary metabolism and the molecular bases of growth traits in this species.
Identifiants
pubmed: 31376663
pii: S1744-117X(19)30140-6
doi: 10.1016/j.cbd.2019.100611
pii:
doi:
Substances chimiques
Fatty Acids, Unsaturated
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
100611Informations de copyright
Copyright © 2019. Published by Elsevier Inc.