Sterilization of Mycobacterium tuberculosis infected samples using methanol preserves anti-tuberculosis drugs for subsequent pharmacological testing studies.
Animals
Antitubercular Agents
/ analysis
Colony Count, Microbial
Feasibility Studies
Female
Laboratory Infection
/ prevention & control
Lung
/ microbiology
Methanol
/ pharmacology
Mice, Inbred BALB C
Microbial Sensitivity Tests
/ methods
Microbial Viability
/ drug effects
Mycobacterium tuberculosis
/ drug effects
Specimen Handling
/ methods
Spectinomycin
/ analogs & derivatives
Stem Cells
/ drug effects
Sterilization
/ methods
Tuberculosis
/ microbiology
Methanol
Mycobacterium-tuberculosis
PK
Pharmacokinetics
Sample sterilization
Journal
Tuberculosis (Edinburgh, Scotland)
ISSN: 1873-281X
Titre abrégé: Tuberculosis (Edinb)
Pays: Scotland
ID NLM: 100971555
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
14
03
2019
revised:
01
06
2019
accepted:
05
06
2019
entrez:
6
8
2019
pubmed:
6
8
2019
medline:
18
2
2020
Statut:
ppublish
Résumé
Pharmacokinetic/pharmacodynamic studies of anti-tuberculosis agents in animal models of tuberculosis are hampered by the frequent necessity to perform sample bioanalysis outside the biosafety level-3 environment. Thus, each specimen has to undergo tedious and time-consuming sample sterilization procedures that may also affect drug stability. Here, we tested treatment of Mycobacterium tuberculosis (Mtb) infected samples with methanol to sterilize samples while preserving drug integrity for further pharmacokinetic/pharmacodynamic evaluations. Tissue samples harvested from Mtb infected mice were homogenized, incubated in methanol, and tested for sterility. Once sterility was confirmed, the samples were used to determine concentrations of the anti-tuberculosis drug spectinamide-1599 in lung homogenates using liquid chromatography coupled with mass spectrometry. The results demonstrate that methanol sterilizes tissue samples harvested from Mtb infected mice without altering the integrity of the drug in the tissue.
Identifiants
pubmed: 31378268
pii: S1472-9792(19)30085-X
doi: 10.1016/j.tube.2019.06.002
pmc: PMC10226109
mid: NIHMS1896088
pii:
doi:
Substances chimiques
Antitubercular Agents
0
spectinamide 1599
0
Spectinomycin
93AKI1U6QF
Methanol
Y4S76JWI15
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
52-55Subventions
Organisme : NIAID NIH HHS
ID : R01 AI120670
Pays : United States
Organisme : NIH HHS
ID : S10 OD016226
Pays : United States
Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.
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