Capsid Structure of a Freshwater Cyanophage Siphoviridae Mic1.

Capsid Proteins / chemistry Cryoelectron Microscopy Microcystis / virology Models, Molecular Protein Conformation Protein Domains Protein Folding Protein Multimerization Siphoviridae / chemistry
capsid assembly cement protein cryo-EM structure cyanophage

Journal

Structure (London, England : 1993)
ISSN: 1878-4186
Titre abrégé: Structure
Pays: United States
ID NLM: 101087697

Informations de publication

Date de publication:
01 10 2019
Historique:
received: 20 03 2019
revised: 11 06 2019
accepted: 12 07 2019
pubmed: 6 8 2019
medline: 21 5 2020
entrez: 6 8 2019
Statut: ppublish

Résumé

Cyanobacteria are the most abundant photosynthetic microorganisms, the global distribution of which is mainly regulated by the corresponding cyanophages. A systematic screening of water samples in the Lake Chaohu enabled us to isolate a freshwater siphocyanophage that infects Microcystis wesenbergii, thus termed Mic1. Using cryoelectron microscopy, we solved the 3.5-Å structure of Mic1 capsid. The major capsid protein gp40 of an HK97-like fold forms two types of capsomers, hexons and pentons. The capsomers interact with each other via the interweaved N-terminal arms of gp40 in addition to a tail-in-mouth joint along the three-fold symmetric axis, resulting in the assembly of capsid in a mortise-and-tenon pattern. The novel-fold cement protein gp47 sticks at the two-fold symmetric axis and further fixes the capsid. These findings provide structural insights into the assembly of cyanophages, and set up a platform to explore the mechanism of specific interactions and co-evolution with cyanobacteria.

Identifiants

DOI: 10.1016/j.str.2019.07.003 PMID: 31378451
pubmed: 31378451
pii: S0969-2126(19)30235-7
doi: 10.1016/j.str.2019.07.003
pii:
doi:

Substances chimiques

Capsid Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1508-1516.e3

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Hua Jin (H)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.

Yong-Liang Jiang (YL)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.

Feng Yang (F)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.

Jun-Tao Zhang (JT)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.

Wei-Fang Li (WF)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.

Ke Zhou (K)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.

Jue Ju (J)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China.

Yuxing Chen (Y)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China. Electronic address: cyxing@ustc.edu.cn.

Cong-Zhao Zhou (CZ)

Hefei National Laboratory for Physical Sciences at the Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China. Electronic address: zcz@ustc.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines nucléocapside › Protéines de capside › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries à Gram négatif photosynthétiques › Cyanobactéries › Microcystis › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure tertiaire des protéines › Domaines protéiques › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Pliage des protéines › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Multimérisation de protéines › Capsid Structure of a Freshwater Cyanophage...
questionsmedicales.fr › Virus › Virus à ADN › Caudovirales › Siphoviridae › Capsid Structure of a Freshwater Cyanophage...