Germ Granules Govern Small RNA Inheritance.
P granules
RNAi
endogenous siRNAs
germ granules
hrde-1
meg-3/4
non-Mendelian inheritance
pptr-1
small RNAs
transgenerational inheritance
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
09 09 2019
09 09 2019
Historique:
received:
13
03
2019
revised:
01
07
2019
accepted:
17
07
2019
pubmed:
6
8
2019
medline:
31
7
2020
entrez:
6
8
2019
Statut:
ppublish
Résumé
In C. elegans nematodes, components of liquid-like germ granules were shown to be required for transgenerational small RNA inheritance. Surprisingly, we show here that mutants with defective germ granules can nevertheless inherit potent small RNA-based silencing responses, but some of the mutants lose this ability after many generations of homozygosity. Animals mutated in pptr-1, which is required for stabilization of P granules in the early embryo, display extraordinarily strong heritable RNAi responses, lasting for tens of generations. Intriguingly, the RNAi capacity of descendants derived from mutants defective in the core germ granule proteins MEG-3 and MEG-4 is determined by the genotype of the ancestors and changes transgenerationally. Further, whether the meg-3/4 mutant alleles were present in the paternal or maternal lineages leads to different transgenerational consequences. Small RNA inheritance, rather than maternal contribution of the germ granules themselves, mediates the transgenerational defects in RNAi of meg-3/4 mutants and their progeny. Accordingly, germ granule defects lead to heritable genome-wide mis-expression of endogenous small RNAs. Upon disruption of germ granules, hrde-1 mutants can inherit RNAi, although HRDE-1 was previously thought to be absolutely required for RNAi inheritance. We propose that germ granules sort and shape the RNA pool, and that small RNA inheritance maintains this activity for multiple generations.
Identifiants
pubmed: 31378614
pii: S0960-9822(19)30934-0
doi: 10.1016/j.cub.2019.07.054
pmc: PMC6739422
pii:
doi:
Substances chimiques
RNA, Helminth
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2880-2891.e4Subventions
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Ltd.. All rights reserved.
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