Selection for antimicrobial resistance is reduced when embedded in a natural microbial community.
Journal
The ISME journal
ISSN: 1751-7370
Titre abrégé: ISME J
Pays: England
ID NLM: 101301086
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
28
02
2019
accepted:
23
07
2019
revised:
22
07
2019
pubmed:
7
8
2019
medline:
31
3
2020
entrez:
7
8
2019
Statut:
ppublish
Résumé
Antibiotic resistance has emerged as one of the most pressing, global threats to public health. In single-species experiments selection for antibiotic resistance occurs at very low antibiotic concentrations. However, it is unclear how far these findings can be extrapolated to natural environments, where species are embedded within complex communities. We competed isogenic strains of Escherichia coli, differing exclusively in a single chromosomal resistance determinant, in the presence and absence of a pig faecal microbial community across a gradient of antibiotic concentration for two relevant antibiotics: gentamicin and kanamycin. We show that the minimal selective concentration was increased by more than one order of magnitude for both antibiotics when embedded in the community. We identified two general mechanisms were responsible for the increase in minimal selective concentration: an increase in the cost of resistance and a protective effect of the community for the susceptible phenotype. These findings have implications for our understanding of the evolution and selection of antibiotic resistance, and can inform future risk assessment efforts on antibiotic concentrations.
Identifiants
pubmed: 31384011
doi: 10.1038/s41396-019-0483-z
pii: 10.1038/s41396-019-0483-z
pmc: PMC6864104
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2927-2937Subventions
Organisme : Medical Research Council
ID : MR/N007174/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
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