Multiscale reverse engineering of the human ocular surface.
Journal
Nature medicine
ISSN: 1546-170X
Titre abrégé: Nat Med
Pays: United States
ID NLM: 9502015
Informations de publication
Date de publication:
08 2019
08 2019
Historique:
received:
31
01
2018
accepted:
21
06
2019
pubmed:
7
8
2019
medline:
7
11
2019
entrez:
7
8
2019
Statut:
ppublish
Résumé
Here we present a miniaturized analog of a blinking human eye to reverse engineer the complexity of the interface between the ocular system and the external environment. Our model comprises human cells and provides unique capabilities to replicate multiscale structural organization, biological phenotypes and dynamically regulated environmental homeostasis of the human ocular surface. Using this biomimetic system, we discovered new biological effects of blink-induced mechanical forces. Furthermore, we developed a specialized in vitro model of evaporative dry-eye disease for high-content drug screening. This work advances our ability to emulate how human physiological systems interface with the external world, and may contribute to the future development of novel screening platforms for biopharmaceutical and environmental applications.
Identifiants
pubmed: 31384041
doi: 10.1038/s41591-019-0531-2
pii: 10.1038/s41591-019-0531-2
pmc: PMC6950645
mid: NIHMS1062057
doi:
Substances chimiques
Glycoproteins
0
lubricin
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1310-1318Subventions
Organisme : NHLBI NIH HHS
ID : DP2 HL127720
Pays : United States
Organisme : NEI NIH HHS
ID : K08 EY025742
Pays : United States
Organisme : NIEHS NIH HHS
ID : P30 ES013508
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY026972
Pays : United States
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