Novel LC-MS/MS method for analysis of metformin and canagliflozin in human plasma: application to a pharmacokinetic study.
Canagliflozin
HPLC–MS/MS
Human plasma
Metformin
Pharmacokinetic study
Journal
BMC chemistry
ISSN: 2661-801X
Titre abrégé: BMC Chem
Pays: Switzerland
ID NLM: 101741142
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
02
09
2017
accepted:
25
06
2019
entrez:
7
8
2019
pubmed:
7
8
2019
medline:
7
8
2019
Statut:
epublish
Résumé
Highly sensitive and selective liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous estimation of the recently approved oral hypoglycemic mixture; metformin (MET) and canagliflozin (CFZ) in human plasma using propranolol HCl (PPL) and tadalafil (TDF) as internal standards (IS), respectively. Analytes were extracted using protein precipitation induced by acetonitrile then liquid-liquid extraction was performed using ethyl acetate. Reversed phase HPLC was carried out using C18 analytical column (50 mm × 4.6 mm i.d., 5 µm) with a simple isocratic mobile phase composed of 0.1% formic acid and acetonitrile (60:40,
Identifiants
pubmed: 31384829
doi: 10.1186/s13065-019-0597-4
pii: 597
pmc: PMC6661773
doi:
Types de publication
Journal Article
Langues
eng
Pagination
82Déclaration de conflit d'intérêts
Competing interestsNot applicable.
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