Urinary miRNA-27b-3p and miRNA-1228-3p correlate with the progression of Kidney Fibrosis in Diabetic Nephropathy.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 08 2019
06 08 2019
Historique:
received:
21
02
2019
accepted:
22
07
2019
entrez:
8
8
2019
pubmed:
8
8
2019
medline:
18
11
2020
Statut:
epublish
Résumé
Diabetic Nephropathy (DN) is a chronic complication of diabetes and the primary cause of end stage renal disease. Differential diagnosis for DN requires invasive histological investigation, thus there is need for non-invasive biomarkers to discriminate among different histological lesions in diabetic patients. With the aim to identify a pattern of differentially expressed miRNAs in kidney biopsies of DN patients, we assayed miRNA expression in kidney biopsies from DN patients, diabetic patients with membranous nephropathy and patients with normal histology. Nine miRNAs were differentially expressed among the three groups, and 2 miRNAs (miR-27b-3p and miR-1228-3p) showed interaction with an ubiquitin-conjugating E2 enzyme variant (UBE2v1). UBE2v1 mediates the formation of lysine 63-linked ubiquitin chains, a mechanism we previously showed as involved in DN kidney fibrosis. Both miRNAs were validated as down-regulated in biopsies and urines of DN patients, possibly affected by DNA methylation. Interestingly, the urinary levels of both miRNAs could also discriminate among different degrees of renal fibrosis. Finally, we showed that the combined urinary expression of both miRNAs was also able to discriminate DN patients from other glomerulonephritides in diabetic patients. In conclusion we identified two miRNAs potentially useful as candidate biomarkers of tubular-interstitial fibrosis in diabetic patients with DN.
Identifiants
pubmed: 31388051
doi: 10.1038/s41598-019-47778-1
pii: 10.1038/s41598-019-47778-1
pmc: PMC6684817
doi:
Substances chimiques
Biomarkers
0
MIRN1228 microRNA, human
0
MIRN27b microRNA, human
0
MicroRNAs
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11357Références
Diabetes Care. 2010 Nov;33(11):2409-15
pubmed: 20671095
Nucleic Acids Res. 2018 Jan 4;46(D1):D239-D245
pubmed: 29156006
FASEB J. 2017 Jan;31(1):308-319
pubmed: 27881486
Nephrol Dial Transplant. 2015 Feb;30(2):155-7
pubmed: 25500333
Br J Cancer. 2015 Jan 20;112(2):365-74
pubmed: 25422913
J Diabetes Metab Disord. 2014 Jun 24;13:69
pubmed: 25028646
Methods. 2001 Dec;25(4):402-8
pubmed: 11846609
Eur J Clin Invest. 2016 Mar;46(3):213-26
pubmed: 26707063
Arthritis Rheum. 2010 May;62(5):1361-71
pubmed: 20131257
Sci Rep. 2016 Feb 03;6:20475
pubmed: 26837280
QJM. 2011 Sep;104(9):771-4
pubmed: 21504987
J Am Soc Nephrol. 2017 Feb;28(2):598-611
pubmed: 27624489
PLoS One. 2013 May 14;8(5):e64184
pubmed: 23691167
J Am Soc Nephrol. 2017 Oct;28(10):2985-2992
pubmed: 28663230
Genome Biol. 2013;14(10):R108
pubmed: 24098934
Acta Diabetol. 2013 Dec;50(6):965-9
pubmed: 23797704
Nucleic Acids Res. 2015 Jan;43(Database issue):D146-52
pubmed: 25378301
J Nephrol. 2013 Sep-Oct;26(5):811-20
pubmed: 23543479
PLoS One. 2013;8(3):e58637
pubmed: 23554909
J Cell Physiol. 2016 Jan;231(1):25-30
pubmed: 26031493
Diabetes. 2018 May;67(5):1013-1023
pubmed: 29453204
Cell. 2001 Jun 15;105(6):711-20
pubmed: 11440714
J Diabetes. 2020 Sep;12(9):633-644
pubmed: 29341487
Int J Clin Exp Pathol. 2015 Jun 01;8(6):6646-55
pubmed: 26261546
Eur J Heart Fail. 2016 Aug;18(8):1000-8
pubmed: 27072074
Nephrol Dial Transplant. 2008 Jun;23(6):1940-5
pubmed: 18156459
J Am Soc Nephrol. 2017 Apr;28(4):1050-1061
pubmed: 28159781
Nucleic Acids Res. 2018 Jan 4;46(D1):D296-D302
pubmed: 29126174
Biomed Res Int. 2013;2013:125469
pubmed: 24089659
Crit Care. 2014 Sep 27;18(5):520
pubmed: 25261195
Mol Cell Endocrinol. 2018 Dec 5;477:90-102
pubmed: 29902497
Clin Sci (Lond). 2016 May;130(9):733-46
pubmed: 26846681
J Assoc Physicians India. 2013 Mar;61(3):194-9
pubmed: 24475681
PLoS One. 2013;8(1):e54662
pubmed: 23358711
J Diabetes Res. 2016;2016:7934504
pubmed: 26798653
Wiley Interdiscip Rev RNA. 2014 Sep-Oct;5(5):697-712
pubmed: 24944010
Am J Kidney Dis. 2002 Apr;39(4):713-20
pubmed: 11920336
Cell. 2010 Apr 2;141(1):129-41
pubmed: 20371350
J Mol Med (Berl). 2018 Jul;96(7):645-659
pubmed: 29806072
Oncotarget. 2016 Jul 26;7(30):47760-47776
pubmed: 27351287
Nat Rev Mol Cell Biol. 2010 Apr;11(4):252-63
pubmed: 20216554
J Am Soc Nephrol. 2010 Mar;21(3):438-47
pubmed: 20056746
World J Diabetes. 2013 Dec 15;4(6):245-55
pubmed: 24379914
J Diabetes Complications. 2016 Apr;30(3):406-14
pubmed: 26775556
Int J Clin Pract. 2009 Aug;63(8):1167-76
pubmed: 18422591
Nephrol Dial Transplant. 2017 Jan 1;32(1):97-110
pubmed: 27190327
Diabetes. 2016 Jan;65(1):216-27
pubmed: 26395742
Nucleic Acids Res. 2013 Jul;41(Web Server issue):W169-73
pubmed: 23680784
Ann N Y Acad Sci. 2015 Sep;1353:72-88
pubmed: 25877817
Ren Fail. 2015 May;37(4):572-5
pubmed: 25682971
Elife. 2015 Aug 12;4:
pubmed: 26267216