Endotracheal tube mucus as a source of airway mucus for rheological study.
cystic fibrosis
muco-obstructive lung disease
mucus
mucus biochemistry
mucus biophysics
Journal
American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229
Informations de publication
Date de publication:
01 10 2019
01 10 2019
Historique:
pubmed:
8
8
2019
medline:
2
4
2020
entrez:
8
8
2019
Statut:
ppublish
Résumé
Muco-obstructive lung diseases (MOLDs), like cystic fibrosis and chronic obstructive pulmonary disease, affect a spectrum of subjects globally. In MOLDs, the airway mucus becomes hyperconcentrated, increasing osmotic and viscoelastic moduli and impairing mucus clearance. MOLD research requires relevant sources of healthy airway mucus for experimental manipulation and analysis. Mucus collected from endotracheal tubes (ETT) may represent such a source with benefits, e.g., in vivo production, over canonical sample types such as sputum or human bronchial epithelial (HBE) mucus. Ionic and biochemical compositions of ETT mucus from healthy human subjects were characterized and a stock of pooled ETT samples generated. Pooled ETT mucus exhibited concentration-dependent rheologic properties that agreed across spatial scales with reported individual ETT samples and HBE mucus. We suggest that the practical benefits compared with other sample types make ETT mucus potentially useful for MOLD research.
Identifiants
pubmed: 31389736
doi: 10.1152/ajplung.00238.2019
pmc: PMC6842913
doi:
Substances chimiques
Cations, Monovalent
0
Polysaccharides
0
Proteins
0
Sodium
9NEZ333N27
Potassium
RWP5GA015D
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
L498-L509Subventions
Organisme : NIDDK NIH HHS
ID : P30 DK065988
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI110098
Pays : United States
Commentaires et corrections
Type : CommentIn
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