SNARE protein SEC22B regulates early embryonic development.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 08 2019
07 08 2019
Historique:
received:
10
01
2019
accepted:
27
06
2019
entrez:
9
8
2019
pubmed:
9
8
2019
medline:
11
11
2020
Statut:
epublish
Résumé
The highly conserved SNARE protein SEC22B mediates diverse and critical functions, including phagocytosis, cell growth, autophagy, and protein secretion. However, these characterizations have thus far been limited to in vitro work. Here, we expand our understanding of the role Sec22b plays in vivo. We utilized Cre-Lox mice to delete Sec22b in three tissue compartments. With a germline deletion of Sec22b, we observed embryonic death at E8.5. Hematopoietic/endothelial cell deletion of Sec22b also resulted in in utero death. Notably, mice with Sec22b deletion in CD11c-expressing cells of the hematopoietic system survive to adulthood. These data demonstrate Sec22b contributes to early embryogenesis through activity both in hematopoietic/endothelial tissues as well as in other tissues yet to be defined.
Identifiants
pubmed: 31391476
doi: 10.1038/s41598-019-46536-7
pii: 10.1038/s41598-019-46536-7
pmc: PMC6685974
doi:
Substances chimiques
R-SNARE Proteins
0
Sec22b protein, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
11434Subventions
Organisme : NCI NIH HHS
ID : R01 CA173878
Pays : United States
Organisme : NHLBI NIH HHS
ID : R35 HL135793
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA203542
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NHLBI NIH HHS
ID : K08 HL128794
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL090775
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL148333
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007863
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL128046
Pays : United States
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