Mouse TMCO5 is localized to the manchette microtubules involved in vesicle transfer in the elongating spermatids.
Aging
/ metabolism
Animals
Antibodies, Monoclonal
/ metabolism
CHO Cells
Cricetinae
Cricetulus
Cytoskeletal Proteins
/ genetics
Golgi Apparatus
/ metabolism
Green Fluorescent Proteins
/ metabolism
Male
Membrane Proteins
/ genetics
Mice
Microtubules
/ metabolism
RNA, Messenger
/ genetics
Spermatids
/ metabolism
Spermatogenesis
Testis
/ metabolism
Transport Vesicles
/ metabolism
Tubulin
/ metabolism
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
23
04
2019
accepted:
25
07
2019
entrez:
9
8
2019
pubmed:
9
8
2019
medline:
11
3
2020
Statut:
epublish
Résumé
As a result of a high-throughput in situ hybridization screening for adult mouse testes, we found that the mRNA for Tmco5 is expressed in round and elongating spermatids. Tmco5 belongs to the Tmco (Transmembrane and coiled-coil domains) gene family and has a coiled-coil domain in the N-terminal and a transmembrane domain in the C-terminal region. A monoclonal antibody raised against recombinant TMCO5 revealed that the protein is expressed exclusively in the elongating spermatids of step 9 to 12 and is localized to the manchette, a transiently emerging construction, which predominantly consists of cytoskeleton microtubules and actin filaments. This structure serves in the transport of Golgi-derived non-acrosomal vesicles. Moreover, induced expression of TMCO5 in CHO cells resulted in the co-localization of TMCO5 with β-tubulin besides the reorganization of the Golgi apparatus. Judging from the results and considering the domain structure of TMCO5, we assume that Tmco5 may have a role in vesicle transport along the manchette.
Identifiants
pubmed: 31393949
doi: 10.1371/journal.pone.0220917
pii: PONE-D-19-11512
pmc: PMC6687282
doi:
Substances chimiques
Antibodies, Monoclonal
0
Cytoskeletal Proteins
0
Membrane Proteins
0
RNA, Messenger
0
Tmco5 protein, mouse
0
Tubulin
0
enhanced green fluorescent protein
0
Green Fluorescent Proteins
147336-22-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0220917Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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