Shear stress modulates inner blood retinal barrier phenotype.
Blood-Retinal Barrier
/ physiology
Capillary Permeability
Cells, Cultured
Claudin-5
/ metabolism
Cytokines
/ metabolism
Endothelium, Vascular
/ metabolism
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique, Indirect
Humans
Phenotype
Retinal Vessels
/ metabolism
Stress, Mechanical
Tight Junctions
/ metabolism
Zonula Occludens-1 Protein
/ metabolism
Blood-retinal barrier
Inflammation
Shear stress
Tight junctions
ZO-1
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
11
01
2019
revised:
28
06
2019
accepted:
05
08
2019
pubmed:
9
8
2019
medline:
15
2
2020
entrez:
9
8
2019
Statut:
ppublish
Résumé
The vascular endothelium responds to the shear stress generated by blood flow and changes function to maintain tissue homeostasis and adapt to injury in pathological conditions. Shear stress in the retinal circulation is altered in patients with retinal vascular diseases, such as diabetic retinopathy. Therefore, we aimed to study the effect of laminar shear stress on barrier properties and on the release of proinflammatory cytokines in human retinal microvascular endothelial cells (HRMEC). HRMEC were cultured in Ibidi flow chambers and exposed to laminar shear stress (0-50 dyn/cm
Identifiants
pubmed: 31394104
pii: S0014-4835(19)30033-8
doi: 10.1016/j.exer.2019.107751
pii:
doi:
Substances chimiques
CLDN5 protein, human
0
Claudin-5
0
Cytokines
0
TJP1 protein, human
0
Zonula Occludens-1 Protein
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107751Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.