Molecular Characterization of Twenty-Five Marine Cyanobacteria Isolated from Coastal Regions of Ireland.
16S rRNA sequence
isoenzyme banding pattern
malate dehydrogenase (MDH)
marine cyanobacteria
molecular characterization
morphology
superoxide dismutase (SOD)
Journal
Biology
ISSN: 2079-7737
Titre abrégé: Biology (Basel)
Pays: Switzerland
ID NLM: 101587988
Informations de publication
Date de publication:
07 Aug 2019
07 Aug 2019
Historique:
received:
28
04
2019
revised:
05
07
2019
accepted:
04
08
2019
entrez:
10
8
2019
pubmed:
10
8
2019
medline:
10
8
2019
Statut:
epublish
Résumé
Twenty-five marine cyanobacteria isolated from Irish coasts were characterized based on their morphological characters and 16S rRNA gene sequence analysis. In addition, superoxide dismutase (SOD) and malate dehydrogenase (MDH) isoenzyme banding patterns were used to differentiate two morphologically ambiguous isolates. In this study, six new cyanobacteria-specific primers were designed, and a 16S rRNA gene of twenty-five morphologically diverse cyanobacteria was successfully PCR amplified (1198-1396 bps). Assembled 16S rRNA sequences were used both for a basic local alignment search tool (BLAST) analysis for genus-level identification and to generate a phylogenetic tree, which yielded two major clusters: One with morphologically homogenous cyanobacteria and the other with morphologically very diverse cyanobacteria.
Identifiants
pubmed: 31394859
pii: biology8030059
doi: 10.3390/biology8030059
pmc: PMC6784279
pii:
doi:
Types de publication
Journal Article
Langues
eng
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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