Function of the lesswright (lwr) gene in the growth, development, and reproduction of Schistosoma japonicum.
Gene silencing
Lesswright
Quantitative RT-PCR
Schistosoma japonicum
Journal
Veterinary parasitology
ISSN: 1873-2550
Titre abrégé: Vet Parasitol
Pays: Netherlands
ID NLM: 7602745
Informations de publication
Date de publication:
Aug 2019
Aug 2019
Historique:
received:
17
04
2019
revised:
12
06
2019
accepted:
13
06
2019
entrez:
10
8
2019
pubmed:
10
8
2019
medline:
7
9
2019
Statut:
ppublish
Résumé
The lesswright (lwr) gene and its products are essential molecules in mitosis, DNA repair, and embryo formation in many eukaryotes. In this study, immunohistochemical analysis revealed that the Lwr protein was located in the internal tissues and the surface layer of the adult Schistosoma japonicum (Sj) worms. The mRNA expression levels of SjLwr at different points were evaluated by quantitative real-time RT-PCR. The expression of SjLwr peaked at 14 days and then decreased thereafter. SjLwr expression was relatively more stable in male worms than in female worms. The functions of SjLwr were explored by siRNA-based gene silencing with a simple soaking method. The results showed that knockdown of the SjLwr gene impaired the growth and development of S. japonicum in mice, as well as survival, morphology, reproductive capacity, and egg vitality. These observations imply that SjLwr presents a novel target for the development of immuno- and/or small molecule-based therapeutics for the control and treatment of schistosome infections.
Identifiants
pubmed: 31395202
pii: S0304-4017(19)30149-9
doi: 10.1016/j.vetpar.2019.06.010
pii:
doi:
Substances chimiques
Helminth Proteins
0
RNA, Small Interfering
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
31-39Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.