Zinc supplementation increases protein titer of recombinant CHO cells.
Chinese Hamster Ovary cells (CHO)
Copper
Erythropoietin (EPO)
IgG
Titer
Zinc
Journal
Cytotechnology
ISSN: 0920-9069
Titre abrégé: Cytotechnology
Pays: United States
ID NLM: 8807027
Informations de publication
Date de publication:
Oct 2019
Oct 2019
Historique:
received:
06
03
2019
accepted:
01
08
2019
pubmed:
10
8
2019
medline:
10
8
2019
entrez:
10
8
2019
Statut:
ppublish
Résumé
In order to study the impact of zinc and copper on the titer levels of mAb and recombinant protein in CHO cells, the IgG-expressing (DP12) and EPO-expressing (SK15) cell lines were cultured in chemically defined media with increasing concentrations of either metal. Supplementation with 25 mg/l in CDM media resulted in a significant increase in EPO (1.7-fold) and IgG (2.6-fold) titers compared to control (no added zinc). Titers at this Zn concentration in CDM containing the insulin replacing agent aurintricarboxylic acid (ATA) (CDM + A) showed a 1.8-fold (EPO) and 1.2-fold (IgG) titers increase compared to control. ATA appeared to also reduce the specific productivity (Qp) enhancement induced by Zn-25, with up to 4.9-fold (DP12) and 1.9-fold (SK15) Qp increase in CDM compared to the 1.6-fold (DP12) and 1.5-fold (SK15) Qp increase observed in CDM + A. A 31% reduced Viable Cell Density (VCD) in DP12 was observed in both Zn-supplemented media (3 × 10
Identifiants
pubmed: 31396753
doi: 10.1007/s10616-019-00334-1
pii: 10.1007/s10616-019-00334-1
pmc: PMC6787129
doi:
Types de publication
Journal Article
Langues
eng
Pagination
915-924Subventions
Organisme : Science Foundation Ireland
ID : [13/IA/1841]
Pays : Ireland
Organisme : Science Foundation Ireland co-funded by ERDF
ID : [12/RC/2275_P2]
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