Integrated magneto-fluorescence nanobeads for ultrasensitive glycoprotein detection using antibody coupled boronate-affinity recognition.
Journal
Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838
Informations de publication
Date de publication:
22 Aug 2019
22 Aug 2019
Historique:
pubmed:
10
8
2019
medline:
28
8
2019
entrez:
10
8
2019
Statut:
ppublish
Résumé
Herein, we report a novel magnet-mediated antibody-boronate sandwich-typed assay (ABSTA) strategy for the ultrasensitive, specific, rapid, and enzyme-free detection of glycoproteins in complex samples. The proposed ABSTA method exhibited ultrahigh sensitivity for HCG with a detection limit of 0.19 mIU mL-1, which is approximately 40-fold lower than that of conventional sandwich enzyme immunoassay.
Substances chimiques
Antibodies, Immobilized
0
Antibodies, Monoclonal
0
Boronic Acids
0
Chorionic Gonadotropin
0
Fluorescent Dyes
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM